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PMID: 1422147 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A 1H-15N NMR study of human c-Ha-ras protein: biosynthetic incorporation of 15N-labeled amino acids.

Journal of biomolecular NMR ·Vol. 2 ·No. 1 ·1992-01-00 ·Pages 71-82

Yamasaki K, Muto Y, Ito Y, Wälchli M, Miyazawa T, Nishimura S, Yokoyama S

Abstract

A 1H-15N NMR study was performed on the GDP-bound form of a truncated human c-Ha-ras oncogene product (171 amino acid residues). Resonance cross peaks of the backbone amide 1H-15N nuclei of a uniformly 15N-labeled protein were observed with heteronuclear single-quantum coherence spectroscopy (HSQC). In order to resolve overlapping cross peaks, selective 15N-labeling of one or two types of amino acid residues (Ala, Arg, Asx, Glx, Gly, His, Ile, Leu, Lys, Met, Phe, Ser, Thr, Tyr and/or Val) was carried out using appropriate E. coli mutant strains. By this procedure, all the backbone 1H-15N cross peaks were classified into amino acid types.

Related Genes
c-Ha-ras
MeSH Terms
Amino Acids/metabolism Cloning, Molecular Escherichia coli/genetics Genes, Synthetic Genes, ras Humans Hydrogen Isotope Labeling/methods Magnetic Resonance Spectroscopy/methods Nitrogen Isotopes Protein Conformation Proto-Oncogene Proteins p21(ras)/biosynthesis,chemistry,genetics Recombinant Proteins/biosynthesis,genetics
Chemicals
Amino Acids Nitrogen Isotopes Recombinant Proteins Hydrogen HRAS protein, human Proto-Oncogene Proteins p21(ras)
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Yamasaki K
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Japan.
Muto Y
Ito Y
Wälchli M
Miyazawa T
Nishimura S
Yokoyama S
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Article Info
Journal
Journal of biomolecular NMR
Abbr.
J Biomol NMR
ISSN
0925-2738
DOI
10.1007/BF02192801
Published
1992-01-00
Pages
71-82
Language
English
Region
Netherlands
NLM ID
9110829
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02192801 View journal details
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