STUDIES ON THE MODE OF ACTION OF DIPHTHERIA TOXIN. II. EFFECT OF TOXIN ON AMINO ACID INCORPORATION IN CELL-FREE SYSTEMS.

The Journal of experimental medicine ·Vol. 120 ·1964-12-01 ·Pages 1019-39

COLLIER RJ, PAPPENHEIMER AM

Abstract

It has been demonstrated that low concentrations of highly purified diphtheria toxin specifically inhibit incorporation of labeled amino acids into polypeptides in extracts from HeLa cells and from rabbit reticulocytes. No inhibition of incorporation occurs in the absence of a specific cofactor. This cofactor has been identified as nicotinamide adenine dinucleotide (NAD). It has been shown that it is one of the steps involving transfer of amino acids from soluble ribonucleic acid to the growing polypeptide chain that is affected by the toxin in the presence of NAD.

Keywords

ALGAE CARBON ISOTOPES CATALYSIS DIPHTHERIA TOXIN EXPERIMENTAL LAB STUDY LEUCINE NAD NADP PEPTIDES PHARMACOLOGY PROTEIN METABOLISM RADIOMETRY RETICULOCYTES RIBONUCLEASE RNA SOLUBLE TISSUE CULTURE TISSUE EXTRACTS TOXICOLOGIC REPORT URACIL NUCLEOTIDES

MeSH Terms

Amino Acids Animals Carbon Isotopes Catalysis Cell-Free System Diphtheria Toxin Eukaryota HeLa Cells Humans Leucine NAD NADP Peptides Pharmacology Proteins/metabolism RNA Rabbits Radiometry Research Reticulocytes Ribonucleases Tissue Culture Techniques Tissue Extracts Toxicology Uracil Nucleotides

Chemicals

Amino Acids Carbon Isotopes Diphtheria Toxin Peptides Proteins Tissue Extracts Uracil Nucleotides NAD NADP RNA Ribonucleases Leucine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

COLLIER R J

PAPPENHEIMER A M

References (16)

16 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.120.6.1019

Published

1964-12-01

Pages

1019-39

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2137798

Subset

External Links

PubMed source DOI: 10.1084/jem.120.6.1019 PMC Full Text View journal details

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STUDIES ON THE MODE OF ACTION OF DIPHTHERIA TOXIN. II. EFFECT OF TOXIN ON AMINO ACID INCORPORATION IN CELL-FREE SYSTEMS.

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