POLYSOMES FROM YEAST: DISTRIBUTION OF MESSENGER RNA AND CAPACITY TO SUPPORT PROTEIN SYNTHESIS IN VITRO.

Science (New York, N.Y.) ·Vol. 147 ·No. 3658 ·1965-02-05 ·Pages 615-7

MARCUS L, BRETTHAUER R, HALVORSON H, BOCK R

Abstract

Individual fractions of polysomes were isolated from yeast. Pulse labeling experiments in vivo show constant specific activity of messenger RNA in each polysome peak; this suggests a uniform density of ribosomes per unit length of messenger RNA. In the cell-free incorporating system, the amount of peptide per ribosome unit increased with the size of polysome.

Keywords

CARBON ISOTOPES EXPERIMENTAL LAB STUDY PHENYLALANINE PROTEIN METABOLISM RIBOSOMES RNA MESSENGER SACCHAROMYCES

MeSH Terms

Carbon Isotopes Escherichia coli In Vitro Techniques Peptides Phenylalanine Polyribosomes Proteins/metabolism RNA RNA, Bacterial RNA, Messenger Research Ribosomes Saccharomyces Saccharomyces cerevisiae

Chemicals

Carbon Isotopes Peptides Proteins RNA, Bacterial RNA, Messenger Phenylalanine RNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

MARCUS L

BRETTHAUER R

HALVORSON H

BOCK R

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.147.3658.615

Published

1965-02-05

Pages

615-7

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.147.3658.615 View journal details

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POLYSOMES FROM YEAST: DISTRIBUTION OF MESSENGER RNA AND CAPACITY TO SUPPORT PROTEIN SYNTHESIS IN VITRO.

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