ONSET AND DURATION OF DNA SYNTHESIS IN ANTIBODY FORMING CELLS AFTER ANTIGEN.

The Journal of experimental medicine ·Vol. 121 ·1965-01-01 ·Pages 125-32

COHEN EP, TALMAGE DW

Abstract

Within 5 hours after the intravenous injection of particulate antigen into the primarily immunized mouse, precursors of antibody-forming cells began DNA synthesis as shown by the incorporation of tritium-labeled thymidine. DNA synthesis continued for at least 24 hours and essentially stopped by 48 hours. No DNA synthesis in antibody-forming precursor cells occurred before the injection of antigen.

Keywords

ANTIBODY FORMATION ANTIGEN-ANTIBODY REACTIONS BCG VACCINATION CELL NUCLEUS DNA EXPERIMENTAL LAB STUDY METABOLISM MICE MICROSCOPY FLUORESCENCE RADIOAUTOGRAPHY THYMIDINE TRITIUM

MeSH Terms

Animals Antibodies Antibody Formation Antigen-Antibody Reactions Antigens Autoradiography BCG Vaccine Cell Division Cell Nucleus DNA DNA Replication Fluorescence Immunoglobulins Metabolism Mice Microscopy Microscopy, Fluorescence Mycobacterium bovis Research Thymidine Tritium Vaccination

Chemicals

Antibodies Antigens BCG Vaccine Immunoglobulins Tritium DNA Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

COHEN E P

TALMAGE D W

References (7)

7 references, click to expand

The cellular basis for antibody formation.
J Cell Physiol Suppl. 1957 Dec;50(Suppl 1):265-301 PMID: 13513691
Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects.
J Exp Med. 1958 Dec 1;108(6):945-56 PMID: 13598821
Agglutinin response, a function of cell number.
J Immunol. 1961 May;86:533-7 PMID: 13734391
Cellular proliferation in relation to antibody synthesis.
Proc Soc Exp Biol Med. 1962 Jan;109:1-4 PMID: 13864631
Autoradiographic studies on the immune response.I. The kinetics of plasma cell proliferation.
J Exp Med. 1962 Jan 1;115:209-30 PMID: 14480461
Transformation of adult allogeneic small lymphocytes after transfusion into newborn rats.
J Exp Med. 1962 May 1;115:997-1008 PMID: 14488083
THE EFFECT OF ENDOTOXIN ON THE ANTIBODY RESPONSE OF TRANSFERRED SPLEEN CELLS.
J Immunol. 1964 Feb;92:223-6 PMID: 14160442

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.121.1.125

Published

1965-01-01

Pages

125-32

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2137966

Subset

External Links

PubMed source DOI: 10.1084/jem.121.1.125 PMC Full Text View journal details

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ONSET AND DURATION OF DNA SYNTHESIS IN ANTIBODY FORMING CELLS AFTER ANTIGEN.

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