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PMID: 14268724 Published · ppublish English Journal Article

ENZYMATIC SYNTHESIS OF TRI- AND TETRANUCLEOTIDES OF DEFINED SEQUENCE.

Science (New York, N.Y.) ·Vol. 148 ·No. 3670 ·1965-04-30 ·Pages 632-4

THACH RE, DOTY P

Abstract

Conditions have been found under which polynucleotide phosphorylase adds only a few nucleotide residues to the 3' end of a dinucleotide primer. Pure tri- and tetranucleotides can be isolated from reaction mixtures in high yield.

Keywords
CARBON ISOTOPES CHEMISTRY CHROMATOGRAPHY EXPERIMENTAL LAB STUDY NUCLEOTIDYLTRANSFERASES POLYNUCLEOTIDES RADIOMETRY
MeSH Terms
Carbon Isotopes Chemical Phenomena Chemistry Chromatography Nucleotides Nucleotidyltransferases Polynucleotides Radiometry Research
Chemicals
Carbon Isotopes Nucleotides Polynucleotides Nucleotidyltransferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
THACH R E
DOTY P
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.148.3670.632
Published
1965-04-30
Pages
632-4
Language
English
Region
United States
NLM ID
0404511
Subset
OM
External Links
PubMed source DOI: 10.1126/science.148.3670.632 View journal details
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