LOCATION AND REGENERATION OF ENTERIOVIRUS RECEPTORS OF HELA CELLS.

Journal of bacteriology ·Vol. 89 ·1965-04-00 ·Pages 1097-100

ZAJAC I, CROWELL RL

Abstract

Zajac, Ihor (Hahnemann Medical College, Philadelphia, Pa.), and Richard L. Crowell. Location and regeneration of enterovirus receptors of HeLa cells. J. Bacteriol. 89:1097-1100. 1965.-Treatment of live HeLa cells with chymotrypsin or trypsin completely inactivated the viral receptors for coxsackievirus B3 and poliovirus T1, respectively. Enzyme-treated cells regained their enterovirus receptor activity after incubation in culture medium. The existence of intracellular receptors for virus could not be demonstrated when live HeLa cells were treated with enzyme prior to disruption and fractionation. These results suggested that receptors for enteroviruses are limited to the cell surface.

Keywords

CHYMOTRYPSIN COXSACKIE VIRUSES CYTOPATHOGENIC EFFECT VIRAL EXPERIMENTAL LAB STUDY HELA CELLS POLIOVIRUS TISSUE CULTURE TRYPSIN

MeSH Terms

Chymotrypsin Cytopathogenic Effect, Viral Enterovirus HeLa Cells Humans Poliovirus Regeneration Research Tissue Culture Techniques Trypsin

Chemicals

Chymotrypsin Trypsin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

ZAJAC I

CROWELL R L

References (10)

10 references, click to expand

EFFECT OF ENZYMES ON THE INTERACTION OF ENTEROVIRUSES WITH LIVING HELA CELLS.
J Bacteriol. 1965 Mar;89:574-82 PMID: 14273631
Direct evidence for the presence of histidine in the active center of chymotrypsin.
Biochemistry. 1963 Mar-Apr;2:252-5 PMID: 13992248
The isolation of microsomal membranes.
Biochem Soc Symp. 1963;22:4-31 PMID: 13975385
Symposium on the biology of cells modified by viruses or antigens. IV. Single-cell techniques in tracing virus-host interactions.
Bacteriol Rev. 1959 Dec;23(4):232-49 PMID: 14420973
The location and nature of enterovirus receptors in susceptible cells.
J Exp Med. 1961 Aug 1;114:161-71 PMID: 13715283
Effect of receptor-destroying enzyme on the growth of EMC virus in tissue culture.
J Immunol. 1958 Jul;81(1):76-81 PMID: 13563882
ELECTROPHORETIC STUDY OF THE INTERACTION OF RADIOACTIVE POLIOVIRUS WITH COMPONENTS OF CULTURED CELLS.
J Bacteriol. 1963 Jun;85:1247-55 PMID: 14047214
The mammalian cell-virus relationship. VI. Sustained infection of HeLa cells by Coxsackie B3 virus and effect on superinfection.
J Exp Med. 1961 Feb 1;113:419-35 PMID: 13696665
Plaque formation and isolation of pure lines with poliomyelitis viruses.
J Exp Med. 1954 Feb;99(2):167-82 PMID: 13130792
Loss of viral receptors in homologous interference by ultraviolet-irradiated Newcastle disease virus.
Virology. 1959 Mar;7(3):315-27 PMID: 13669300

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.89.4.1097-1100.1965

Published

1965-04-00

Pages

1097-100

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC277602

Subset

External Links

PubMed source DOI: 10.1128/jb.89.4.1097-1100.1965 PMC Full Text View journal details

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