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PMID: 14292989 Published · ppublish English Journal Article

PREPARATION AND PROPERTIES OF A VI ANTIGEN-DEGRADING ENZYME.

Journal of bacteriology ·Vol. 89 ·1965-05-00 ·Pages 1217-24

BAKER EE, WHITESIDE RE

Abstract

Baker, Edgar E. (Boston University School of Medicine, Boston, Mass.), and Roberta E. Whiteside. Preparation and properties of a Vi antigen-degrading enzyme. J. Bacteriol. 89:1217-1224. 1965.-Vi antigen can be hydrolyzed by an inducible enzyme produced by a microorganism isolated from soil. The organism can be identified as Bacillus sphaericus. The enzyme in the culture supernatant fluid can be concentrated and partially purified by precipitation with ammonium sulfate at 50% of saturation. The partially purified enzyme is relatively stable. Incubation of the enzyme with Vi antigen causes a rapid loss of serological activity, a decrease in viscosity, the loss of ability to precipitate albumin at pH 4.0, and the production of reducing sugar and hexosamine. Optimal conditions for enzyme action on Vi antigen are pH 8.4 and 40 C. The reaction rate is independent of the substrate concentration. Intact protein and calcium activates the Vi antigen-enzyme system. The enzyme is not inhibited by its reaction products.

Keywords
BACILLUS ENZYME INHIBITORS ENZYMES EXPERIMENTAL LAB STUDY HEMAGGLUTINATION INHIBITION TESTS HEXOSAMINES HYDROGEN-ION CONCENTRATION POLYSACCHARIDES BACTERIAL PRECIPITATION PRECIPITIN TESTS SALMONELLA TYPHOSA SERUM ALBUMIN SOIL MICROBIOLOGY TEMPERATURE VISCOSITY
MeSH Terms
Bacillus Chemical Precipitation Enzyme Inhibitors Enzymes Hemagglutination Inhibition Tests Hexosamines Hydrogen-Ion Concentration Molecular Weight Polysaccharides Polysaccharides, Bacterial Precipitin Tests Research Salmonella typhi Serum Albumin Soil Microbiology Temperature Viscosity
Chemicals
Enzyme Inhibitors Enzymes Hexosamines Polysaccharides Polysaccharides, Bacterial Serum Albumin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
BAKER E E
WHITESIDE R E
References (17)
17 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.89.5.1217-1224.1965
Published
1965-05-00
Pages
1217-24
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC277631
Subset
OM
External Links
PubMed source DOI: 10.1128/jb.89.5.1217-1224.1965 PMC Full Text View journal details
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