Home LiteratureArticle Details
PMID: 14293012 Published · ppublish English Journal Article

LYSIS OF BACTERIAL PROTOPLASTS AND SPHEROPLASTS BY STAPHYLOCOCCAL ALPHA-TOXIN AND STREPTOLYSIN S.

Journal of bacteriology ·Vol. 89 ·1965-05-00 ·Pages 1387-92

BERNHEIMER AW, SCHWARTZ LL

Abstract

Bernheimer, Alan W. (New York University School of Medicine, New York, N.Y.), and Lois L. Schwartz. Lysis of bacterial protoplasts and spheroplasts by staphylococcal alpha-toxin and streptolysin S. J. Bacteriol. 89:1387-1392. 1965.-Protoplasts of Bacillus megaterium, Sarcina lutea, and Streptococcus pyogenes, and spheroplasts of Escherichia coli were lysed by staphylococcal alpha-toxin, whereas spheroplasts of Vibrio metschnikovii and V. comma were not. In the spectrum of its lytic action, streptolysin S qualitatively resembled staphylococcal alpha-toxin except for failure to lyse S. pyogenes protoplasts. In contrast to the two foregoing agents, streptolysin O did not lyse protoplasts and spheroplasts. The observations are interpreted in relation to similarities and differences in lipid composition of bacterial and mammalian cell membranes.

Keywords
BACILLUS MEGATERIUM BACTERIOLYSIS CELL MEMBRANE PERMEABILITY ESCHERICHIA COLI EXPERIMENTAL LAB STUDY MURAMIDASE PENICILLIN PHARMACOLOGY PROTOPLASTS SARCINA STAPHYLOCOCCUS STREPTOCOCCUS PYOGENES STREPTOLYSIN TOXINS AND ANTITOXINS VIBRIO
MeSH Terms
Animals Antitoxins Bacillus megaterium Bacterial Proteins Bacterial Toxins Bacteriolysis Cell Membrane Permeability Escherichia coli Hemolysin Proteins Muramidase Penicillins Pharmacology Protoplasts Research Sarcina Spheroplasts Staphylococcus Streptococcus pyogenes Streptolysins Toxins, Biological Vibrio
Chemicals
Antitoxins Bacterial Proteins Bacterial Toxins Hemolysin Proteins Penicillins Streptolysins Toxins, Biological staphylococcal alpha-toxin streptolysin O streptolysin S Muramidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
BERNHEIMER A W
SCHWARTZ L L
References (16)
16 references, click to expand
  1. The localization of alkaline phosphatase in E. coli K12.
    Biochem Biophys Res Commun. 1961 Jun 2;5:104-8 PMID: 13765699
  2. EFFECT OF STAPHYLOCOCCAL AND OTHER BACTERIAL TOXINS ON PLATELETS IN VITRO.
    J Pathol Bacteriol. 1965 Jan;89:209-23 PMID: 14263463
  3. Pneumococcal Hemolysin: The Preparation of Concentrates, and Their Action on Red Cells.
    J Bacteriol. 1942 May;43(5):607-27 PMID: 16560525
  4. Studies on the bacteriophages of hemolytic streptococci. II. Antigens released from the streptococcal cell wall by a phage-associated lysin.
    J Exp Med. 1958 Dec 1;108(6):803-21 PMID: 13598814
  5. Some changes in the surface structure of gram-negative bacteria induced by penicillin action.
    Nature. 1958 May 10;181(4619):1321-4 PMID: 13541461
  6. Release of compounds containing diaminopimelic acid from Vibrio metchnikovi treated with antibody and complement.
    Nature. 1959 Oct 24;184(Suppl 17):1341-2 PMID: 14445361
  7. The association of lipoproteins with the inhibition of streptolysin S by serum.
    J Clin Invest. 1950 Dec;29(12):1636-45 PMID: 14794795
  8. Isolation and composition of staphylococcal alpha toxin.
    J Gen Microbiol. 1963 Mar;30:455-68 PMID: 13967637
  9. Loss of myocardial contractility induced in isolated mammalian hearts by streptolysin O.
    J Exp Med. 1956 Sep 1;104(3):361-73 PMID: 13357690
  10. The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme.
    J Bacteriol. 1953 Dec;66(6):688-95 PMID: 13117795
  11. Synthesis of capsular polysaccharide (hyaluronic acid) by protoplastmembrane preparations of group A Streptococcus.
    J Biol Chem. 1962 Feb;237:273-9 PMID: 14470033
  12. BACTERIAL PROTOPLASTS INDUCED BY PENICILLIN.
    Proc Natl Acad Sci U S A. 1956 Sep;42(9):574-7 PMID: 16589908
  13. STUDIES ON LYSOSOMES. III. THE EFFECTS OF STREPTOLYSINS O AND S ON THE RELEASE OF ACID HYDROLASES FROM A GRANULAR FRACTION OF RABBIT LIVER.
    J Exp Med. 1963 Aug 1;118:205-22 PMID: 14074385
  14. Lysosomal disruption by bacterial toxins.
    J Bacteriol. 1964 May;87(5):1100-4 PMID: 5874534
  15. Lysis of gram-negative bacteria by lysozyme.
    Biochim Biophys Acta. 1956 Oct;22(1):189-91 PMID: 13373865
  16. The inhibitory effects of cholesterol and related sterols on haemolysis by streptolysin O.
    Br J Exp Pathol. 1953 Apr;34(2):174-80 PMID: 13051526
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.89.5.1387-1392.1965
Published
1965-05-00
Pages
1387-92
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC277656
Subset
OM
External Links
PubMed source DOI: 10.1128/jb.89.5.1387-1392.1965 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2