ENZYMATIC DEACYLATION OF S35-BENZYLPENICILLIN.

Journal of bacteriology ·Vol. 90 ·1965-08-00 ·Pages 380-3

PRUESS DL, JOHNSON MJ

Abstract

Pruess, David L. (University of Wisconsin, Madison), and Marvin J. Johnson. Enzymatic deacylation of S(35)-benzylpenicillin. J. Bacteriol. 90:380-383. 1965.-S(35)-benzylpenicillin, penicilloic acid, and penilloic acid were deacylated by cell suspensions of Escherichia coli and Micrococcus roseus. Both cultures deacylated penicillin most rapidly and penilloic acid least rapidly. The deacylase activity of M. roseus against penicilloic acid was cell-bound, probably requiring a metal ion for activity.

Keywords

ACETONE BUFFERS CENTRIFUGATION CHEMISTRY CHROMATOGRAPHY CULTURE MEDIA ESCHERICHIA COLI EXPERIMENTAL LAB STUDY FERMENTATION HYDROGEN-ION CONCENTRATION METABOLISM MICROCOCCUS PENICILLIN G PHARMACOLOGY PHENYLACETATES RADIOMETRY SULFUR ISOTOPES

MeSH Terms

Acetone Acylation Buffers Centrifugation Chemical Phenomena Chemistry Chromatography Culture Media Escherichia coli Fermentation Hydrogen-Ion Concentration Metabolism Micrococcus Penicillanic Acid Penicillin G Penicillins Pharmacology Phenylacetates Radiometry Research Sulfur Isotopes

Chemicals

Buffers Culture Media Penicillins Phenylacetates Sulfur Isotopes penicilloic acid Acetone Penicillanic Acid penilloic acid Penicillin G

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

PRUESS D L

JOHNSON M J

References (13)

13 references, click to expand

Distribution and substrate specificity of benzylpenicillin acylase.
Appl Microbiol. 1963 Jan;11:1-6 PMID: 13955341
PENICILLIN AMIDASE FROM COLIFORMS: ITS EXTRACTION AND SOME CHARACTERISTICS.
Nature. 1964 Feb 22;201:824 PMID: 14161218
PRODUCTION OF PENICILLIN ACYLASE.
Appl Microbiol. 1964 May;12:185-7 PMID: 14170952
VARIETY OF SUBSTRATES FOR A BACTERIAL BENZYL PENICILLIN-SPLITTING ENZYME.
Nature. 1964 Aug 1;203:520 PMID: 14202374
Reactions of 6-aminopenicillanic acid with carbohydrates and related substances.
Biochem J. 1964 Sep;92(3):643-8 PMID: 4953772
INTERMEDIATES IN INORGANIC SULFATE UTILIZATION BY PENICILLIUM CHRYSOGENUM.
Arch Biochem Biophys. 1963 Nov;103:216-26 PMID: 14084584
Sulfate utilization by penicillin-producing mutants of Penicillium chrysogenum.
J Bacteriol. 1958 Oct;76(4):400-5 PMID: 13587479
The distribution of sulfur-containing compounds in penicillin fermentations.
J Biol Chem. 1959 Jul;234(7):1850-6 PMID: 13672975
SPECIFICITY OF PENICILLIN AMIDASES.
Proc Soc Exp Biol Med. 1963 Aug-Sep;113:1008-12 PMID: 14073094
Comparative study of the binding of synnematin and penicillin by bacterial cells.
J Bacteriol. 1958 Oct;76(4):385-92 PMID: 13587477
PROPERTIES OF THE PENICILLIN DEACYLASE ENZYME OF ESCHERICHIA COLI.
Nature. 1964 Aug 1;203:519-20 PMID: 14202373
The nature of the binding of penicillin by bacterial cells.
J Bacteriol. 1956 Jan;71(1):84-90 PMID: 13286234
Activity and properties of para-aminobenzyl penicillin.
Appl Microbiol. 1953 Jul;1(4):163-6 PMID: 13066024

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.90.2.380-383.1965

Published

1965-08-00

Pages

380-3

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC315654

Subset

External Links

PubMed source DOI: 10.1128/jb.90.2.380-383.1965 PMC Full Text View journal details

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