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PMID: 14331189 Published · ppublish English Journal Article

POLYRIBOSOMES FROM ESCHERICHIA COLI: ENZYMATIC METHOD FOR ISOLATION.

Science (New York, N.Y.) ·Vol. 149 ·No. 3684 ·1965-08-06 ·Pages 647-9

DRESDEN M, HOAGLAND MB

Abstract

Polyribosomes can be rapidly extracted from Escherichia coli by sequential passage of the cells through solutions of a chelating agent and lysozyme in a centrifugal field. Biosynthesis of protein in whole cells and in a cell-free system occurs almost exclusively in the polyribosomes.

Keywords
BIOCHEMISTRY EDTA ENDOPLASMIC RETICULUM ESCHERICHIA COLI MURAMIDASE RIBOSOMES
MeSH Terms
Animals Biochemical Phenomena Biochemistry Cell-Free System Edetic Acid Endoplasmic Reticulum Escherichia coli Muramidase Polyribosomes Ribosomes
Chemicals
Edetic Acid Muramidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
DRESDEN M
HOAGLAND M B
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.149.3684.647
Published
1965-08-06
Pages
647-9
Language
English
Region
United States
NLM ID
0404511
Subset
OM
External Links
PubMed source DOI: 10.1126/science.149.3684.647 View journal details
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