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PMID: 14340060 Published · ppublish English Journal Article

THE ADENOSINE-TRIPHOSPHATASE SYSTEM RESPONSIBLE FOR CATION TRANSPORT IN ELECTRIC ORGAN: EXCLUSION OF PHOSPHOLIPIDS AS INTERMEDIATES.

The Biochemical journal ·Vol. 94 ·1965-03-00 ·Pages 692-9

GLYNN IM, SLAYMAN CW, EICHBERG J, DAWSON RM

Abstract

1. Subcellular fractions were prepared from the electric organs of Electrophorus and Torpedo and assayed for adenosine-triphosphatase activity. 2. Treatment of the ;low-speed' fraction from Torpedo with m-urea gave an adenosine-triphosphatase preparation that was almost completely (98%) inhibited by ouabain (0.1mg./ml.) and dependent on the simultaneous presence of Na(+) and K(+). 3. The adenosine-triphosphatase preparations were exposed to [gamma-(32)P]ATP for 30sec. in the presence of (i) Na(+), (ii) K(+), (iii) Na(+)+K(+) and (iv) Na(+)+K(+)+ouabain. No significant labelling of phosphatidic acid, triphosphoinositide or any other phospholipid was observed. 4. The results suggest that phospholipids do not act as phosphorylated intermediates in the ;transport adenosine-triphosphatase' system of electric organ.

Keywords
ADENOSINE TRIPHOSPHATASE BIOLOGICAL TRANSPORT CELL MEMBRANE PERMEABILITY EELS ELECTRIC ORGAN ELECTRICITY EXPERIMENTAL LAB STUDY IONS METABOLISM MUSCLES OUABAIN PHARMACOLOGY PHOSPHOLIPIDS POTASSIUM SODIUM UREA
MeSH Terms
Adenosine Adenosine Triphosphatases Animals Biological Transport Cations Cell Membrane Permeability Eels Electric Organ Electricity Ions Metabolism Muscles Ouabain Pharmacology Phospholipids Potassium Research Sodium Urea
Chemicals
Cations Ions Phospholipids Ouabain Urea Sodium Adenosine Triphosphatases Adenosine Potassium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
GLYNN I M
SLAYMAN C W
EICHBERG J
DAWSON R M
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25 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj0940692
Published
1965-03-00
Pages
692-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1206604
Subset
OM
External Links
PubMed source DOI: 10.1042/bj0940692 PMC Full Text View journal details
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