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PMID: 14340070 Published · ppublish English Journal Article

THE OCCURRENCE OF APIOSE IN LEMNA (DUCKWEED) AND OTHER ANGIOSPERMS.

The Biochemical journal ·Vol. 94 ·1965-03-00 ·Pages 768-72

DUFF RB

Abstract

1. The branched-chain pentose apiose reacts with the benzidine-trichloroacetic acid reagent on paper chromatograms to give a yellow spot with.an intense white fluorescence in ultraviolet light; on chromatograms developed with butanol-acetic acid-water this spot lies between fucose and rhamnose. 2. Examination of paper chromatograms of hydrolysates of whole plant material has shown the presence of a substance with these properties in a wide variety of species. 3. Among the plants examined two members of the Lemnaceae (Lemna minor and Wolffia arrhiza) were found to be especially rich sources, comparable with Posidonia australis (Bell, Hardwick, Isherwood & Cahn, 1954). 4. Measurements of the apiose content of fractions derived from Lemna have shown that the sugar is present at a concentration of about 4% in the holocellulose, and part of this is retained in the alpha-cellulose left by extraction with 24% (w/v) potassium hydroxide containing borate.

Keywords
BIOCHEMISTRY CHEMISTRY ANALYTICAL CHROMATOGRAPHY EXPERIMENTAL LAB STUDY PENTOSES PLANTS POLYSACCHARIDES
MeSH Terms
Araceae Biochemical Phenomena Biochemistry Cellulose Chemistry Techniques, Analytical Chromatography Chromatography, Paper Pentoses Plants Polysaccharides Research
Chemicals
Pentoses Polysaccharides Cellulose apiose
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
DUFF R B
References (5)
5 references, click to expand
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  2. Detection of sugars on paper chromatograms.
    Nature. 1950 Sep 9;166(4219):444-5 PMID: 14775715
  3. Filter-paper chromatography: extraction of sugars from the paper at room temperature.
    Nature. 1950 Sep 16;166(4220):476-7 PMID: 14780097
  4. Use of the arsenomolybdate-somogyi reagent in quantitative paper chromatography, and its application to the study of sucrose utilization by a fungus.
    Nature. 1950 May 27;165(4204):848-49 PMID: 15423480
  5. Filter-paper partition chromatography of sugars: 1. General description and application to the qualitative analysis of sugars in apple juice, egg white and foetal blood of sheep. with a note by R. G. Westall.
    Biochem J. 1948;42(2):238-50 PMID: 16748273
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Published
1965-03-00
Pages
768-72
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1206615
Subset
OM
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