A FRACTIONATION OF THE HISTONES OF GROUP F2A FROM CALF THYMUS.

The Biochemical journal ·Vol. 94 ·1965-01-00 ·Pages 127-30

PHILLIPS DM, JOHNS EW

Abstract

1. The calf-thymus histone group F2a has been separated into two subfractions by stepwise precipitation with acetone from acid solution. 2. Carboxymethyl-cellulose and dextran-gel column chromatography and a method involving dialysis against ethanol have also given the subfractions, but the acetone-hydrochloric acid method has proved to be the most practicable. 3. The two subfractions differ significantly in their amino acid composition and in the pattern of peptides obtained by tryptic digestion. Both fractions have a very low content of N-terminal amino acids and contain acetyl groups.

Keywords

AMINO ACIDS BIOCHEMISTRY CATTLE CHEMISTRY ANALYTICAL CHROMATOGRAPHY DEXTRAN DIALYSIS ELECTROPHORESIS EXPERIMENTAL LAB STUDY HISTONES METHYLCELLULOSE THYMUS GLAND

MeSH Terms

Amino Acids Animals Biochemical Phenomena Biochemistry Cattle Chemistry Techniques, Analytical Chromatography Chromatography, Gel Dextrans Dialysis Electrophoresis Fluid Therapy Histones Methylcellulose Renal Dialysis Research Solutions Thymus Gland

Chemicals

Amino Acids Dextrans Histones Solutions Methylcellulose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

PHILLIPS D M

JOHNS E W

References (12)

12 references, click to expand

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Identification of some peptides from an arginine-rich histone and their bearing on the structure of deoxyribonucleohistone.
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A micromethod for the determination of N- and O-acetyl groups.
Biochem J. 1963 Mar;86:397-401 PMID: 13943141
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Biochem J. 1963 May;87:258-63 PMID: 13943142
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj0940127

Published

1965-01-00

Pages

127-30

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1206417

Subset

External Links

PubMed source DOI: 10.1042/bj0940127 PMC Full Text View journal details

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A FRACTIONATION OF THE HISTONES OF GROUP F2A FROM CALF THYMUS.

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