PHOSPHATIDIC ACID SYNTHESIS IN YEAST.

The Biochemical journal ·Vol. 94 ·1965-01-00 ·Pages 240-6

KUHN NJ, LYNEN F

Abstract

1. The presence of palmitoyl-CoA-l-glycerol 1-phosphate palmitoyltransferase (EC2.3.1.15) has been demonstrated in a particulate fraction of baker's yeast. 2. The enzyme has been characterized, and its activity studied as a function of pH and concentration of substrates. 3. Inhibition by thiol poisons and protection by acyl-CoA have been used to obtain information on the active site. 4. By various methods of supplying acyl radicals, the species ;palmitoyl-CoA' has been shown to be the true acyl donor to the transferase.

Keywords

ACYLTRANSFERASES AMIDES CATALYSIS CHROMATOGRAPHY COENZYME A ENZYME INHIBITORS ETHYLMALEIMIDE EXPERIMENTAL LAB STUDY GLYCEROPHOSPHATES HYDROGEN-ION CONCENTRATION LIGASES OLEIC ACID PALMITIC ACID PHARMACOLOGY PHOSPHOLIPID METABOLISM PYRROLES SACCHAROMYCES

MeSH Terms

Acyl Coenzyme A Acyltransferases Amides Catalysis Chromatography Coenzyme A Enzyme Inhibitors Ethylmaleimide Glycerophosphates Hydrogen-Ion Concentration Ligases Lipid Metabolism Oleic Acid Oleic Acids Palmitic Acid Palmitoyl Coenzyme A Pharmacology Phosphatidic Acids Phospholipids/metabolism Pyrroles Research Saccharomyces

Chemicals

Acyl Coenzyme A Amides Enzyme Inhibitors Glycerophosphates Oleic Acids Phosphatidic Acids Phospholipids Pyrroles Palmitoyl Coenzyme A Oleic Acid Palmitic Acid alpha-glycerophosphoric acid Acyltransferases Ligases Ethylmaleimide Coenzyme A

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

KUHN N J

LYNEN F

References (18)

18 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj0940240

Published

1965-01-00

Pages

240-6

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1206433

Subset

External Links

PubMed source DOI: 10.1042/bj0940240 PMC Full Text View journal details

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