THE MAGNESIUM-ION-DEPENDENT CLEAVAGE OF THE VINYL ETHER LINKAGE OF BRAIN ETHANOLAMINE PLASMALOGEN.

The Biochemical journal ·Vol. 94 ·1965-01-00 ·Pages 252-8

ANSELL GB, SPANNER S

Abstract

1. There was a significant decrease in the amount of endogenous ethanolamine phospholipids when preparations of whole brain were incubated in bicarbonate-Ringer solutions, leading in particular to the hydrolysis of vinyl ether groups. 2. The hydrolysis of ethanolamine phospholipids in such preparations was abolished in the absence of bivalent cations. 3. An enzyme present in extracts of acetone-dried brain powders that cleaved the vinyl ether linkage in ethanolamine plasmalogen maximally at pH 7.4 required Mg(2+) for activity. 4. The cleavage of the vinyl ether linkage of an ethanolamine lysoplasmalogen was enhanced in the presence of Mg(2+) but the requirement was not absolute.

Keywords

BRAIN CHEMISTRY CALCIUM DIALYSIS EXPERIMENTAL LAB STUDY HYDROGEN-ION CONCENTRATION IONS MAGNESIUM MANGANESE PHARMACOLOGY PHOSPHOLIPASE PHOSPHOLIPID METABOLISM PHOSPHOLIPIDS PLASMALOGENS RATS VINYL ETHER

MeSH Terms

Brain Brain Chemistry Calcium Dialysis Ethanolamines Hydrogen-Ion Concentration Hydrolysis Ions Magnesium Manganese Pharmacology Phosphatidylethanolamines Phospholipases Phospholipids/metabolism Plasmalogens Rats Renal Dialysis Research Vinyl Compounds

Chemicals

Ethanolamines Ions Phosphatidylethanolamines Phospholipids Plasmalogens Vinyl Compounds phosphatidal ethanolamines vinyl ether phosphatidylethanolamine Manganese Phospholipases Magnesium Calcium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

ANSELL G B

SPANNER S

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31 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj0940252

Published

1965-01-00

Pages

252-8

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1206435

Subset

External Links

PubMed source DOI: 10.1042/bj0940252 PMC Full Text View journal details

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THE MAGNESIUM-ION-DEPENDENT CLEAVAGE OF THE VINYL ETHER LINKAGE OF BRAIN ETHANOLAMINE PLASMALOGEN.

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