Correlations between plasma protein fractions, antibody titers, and the passive transfer of delayed and immediate cutaneous reactivity to tuberculin PPD and tuberculopolysaccharides.

The Journal of experimental medicine ·Vol. 101 ·No. 4 ·1955-04-01 ·Pages 391-420

COLE LR, FAVOUR CB

Abstract

GUINEA PIGS SENSITIZED WITH TUBERCLE BACILLI DEMONSTRATE A DUAL ALLERGIC RESPONSE MEDIATED BY TWO CHEMICALLY DISTINCT PLASMA FRACTIONS: 1. Antibody to tuberculopolysaccharide is located exclusively in fraction II (gamma globulin). This fraction will passively transfer systemic anaphylaxis and urticarial type skin reactivity to tuberculopolysaccharide, and contains the Middlebrook-Dubos antibody. 2. Antibody to tuberculoprotein is contained exclusively in a new plasma fraction called fraction IV-10. By Cohn's Method X, fraction IV-10 is a part of fraction IV (alpha globulin) and to a lesser extent V (albumin). This fraction will passively transfer to normal guinea pigs a delayed type skin sensitivity to tuberculin PPD which is maximal between 18 and 30 hours, and it contains the Boyden antibody. When fractions II and IV-10 are combined, the antibody to tuberculopolysaccharide inhibits the passive transfer of delayed type reactivity. Combination of these two fractions does not alter their separate in vitro hemagglutinating properties. Adsorption of IV-10 with Boyden sensitized cells removes its ability to transfer delayed type tuberculin sensitivity. Adsorption of II with Middlebrook-Dubos-sensitized cells removes its capacity to effect passive transfer of immediate type reactivity to tuberculopolysaccharides.

Keywords

ANTIGENS AND ANTIBODIES BLOOD PROTEINS POLYSACCHARIDES TUBERCULIN REACTION

MeSH Terms

Animals Antibodies Antigens Blood Proteins Guinea Pigs Polysaccharides Serum Albumin Serum Albumin, Human Serum Globulins Tuberculin Tuberculin Test

Chemicals

Antibodies Antigens Blood Proteins Polysaccharides Serum Albumin Serum Globulins Tuberculin plasma protein fraction Serum Albumin, Human

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

COLE L R

FAVOUR C B

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22 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.101.4.391

Published

1955-04-01

Pages

391-420

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2136478

Subset

External Links

PubMed source DOI: 10.1084/jem.101.4.391 PMC Full Text View journal details

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Correlations between plasma protein fractions, antibody titers, and the passive transfer of delayed and immediate cutaneous reactivity to tuberculin PPD and tuberculopolysaccharides.

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