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PMID: 1438273 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The anticodon triplet is not sufficient to confer methionine acceptance to a transfer RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 22 ·1992-11-15 ·Pages 10768-71

Senger B, Despons L, Walter P, Fasiolo F

Abstract

Previous work suggested that the presence of the anticodon CAU alone was enough to confer methionine acceptance to a tRNA. Conversions of Escherichia coli nonmethionine tRNAs to a methionine-accepting species were obtained by substitutions reconstructing the whole methionine anticodon loop together with preservation (or introduction) of the acceptor stem base A73. We show here that the CAU triplet alone is unable to confer methionine acceptance when transplanted into a yeast aspartic tRNA. Both non-anticodon bases of the anticodon loop of yeast tRNA(Met) and A73 are required in addition to CAU for methionine acceptance. The importance of these non-anticodon bases in other CAU-containing tRNA frameworks was also established. These specific non-anticodon base interactions make a substantial thermodynamic contribution to the methionine acceptance of a transfer RNA.

MeSH Terms

Anticodon/genetics,metabolism Base Sequence Kinetics Methionine/metabolism Models, Structural Molecular Sequence Data Nucleic Acid Conformation RNA, Transfer, Met/genetics,metabolism Saccharomyces cerevisiae/genetics Transcription, Genetic

Chemicals

Anticodon RNA, Transfer, Met Methionine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Senger B

Laboratoire de Biochimie, Centre National de la Recherche Scientifique, Strasbourg, France.

Despons L

Walter P

Fasiolo F

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24 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.89.22.10768

Published

1992-11-15

Pages

10768-71

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC50423

Subset

External Links

PubMed source DOI: 10.1073/pnas.89.22.10768 PMC Full Text View journal details

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The anticodon triplet is not sufficient to confer methionine acceptance to a transfer RNA.

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