A diazo coupling method for the electron microscopic localization of cholinesterase.

The Journal of biophysical and biochemical cytology ·Vol. 6 ·1959-12-00 ·Pages 399-406

LEHRER GM, ORNSTEIN L

Abstract

The presence of cholinesterase at the myoneural junction of intercostal muscle has been demonstrated in both light and electron microscopic preparations. A new simultaneous diazo coupling technique using alpha-naphthyl acetate as substrate and "hexazonium pararosanilin" as coupler has been applied to cold formalin-fixed tissues. After postfixation in buffered osmium tetroxide the sites of esterase activity are faithfully demonstrated at a high level of resolution. The details of cholin-esterase distribution and some technical aspects of the procedure are discussed.

Keywords

CHOLINESTERASE/chemistry

MeSH Terms

Biological Phenomena Cholinesterases/chemistry Electrons Esterases Microscopy, Electron Naphthols Neuromuscular Junction Osmium Tetroxide

Chemicals

Naphthols alpha-naphthyl acetate Esterases Cholinesterases Osmium Tetroxide

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

LEHRER G M

ORNSTEIN L

References (17)

17 references, click to expand

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Article Info

Journal

The Journal of biophysical and biochemical cytology

Abbr.

J Biophys Biochem Cytol

ISSN

0095-9901

DOI

10.1083/jcb.6.3.399

Published

1959-12-00

Pages

399-406

Language

English

Region

United States

NLM ID

17840020R

PMCID

PMC2224705

Subset

External Links

PubMed source DOI: 10.1083/jcb.6.3.399 PMC Full Text

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A diazo coupling method for the electron microscopic localization of cholinesterase.

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