Purification of staphylococcal alpha-hemolysin.

Journal of bacteriology ·Vol. 83 ·1962-04-00 ·Pages 914-8

MADOFF MA, WEINSTEIN L

Abstract

Madoff, Morton A. (New England Center Hospital, Boston, Mass.) and Louis Weinstein. Purification of staphylococcal alpha-hemolysin. J. Bacteriol. 83:914-918. 1962. - Staphylococcal alpha-toxin has been purified by metal-ion precipitation, column chromatography, and continuous-flow paper electrophoresis. The resultant preparation is highly unstable. A single line is obtained on agar-gel diffusion analysis against staphylococcal antiserum. Two bands, both associated with hemolytic activity and migrating cathodally at pH 8.4, are obtained on starch-gel electrophoresis. Levels of lytic, dermonecrotic, and lethal toxin activity maintain a constant relationship in the crude and purified materials, suggesting the unity of these effects.

Keywords

STAPHYLOCOCCUS TOXINS AND ANTITOXINS/chemistry

MeSH Terms

Antitoxins/chemistry Bacterial Toxins Electrodes Exotoxins Hemolysin Proteins Hospitals Molecular Weight Staphylococcus Toxins, Biological/chemistry

Chemicals

Antitoxins Bacterial Toxins Exotoxins Hemolysin Proteins Toxins, Biological staphylococcal alpha-toxin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

MADOFF M A

WEINSTEIN L

References (10)

10 references, click to expand

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Starch-gel electrophoresis of anterior pituitary hormones.
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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.83.4.914-918.1962

Published

1962-04-00

Pages

914-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC279374

Subset

External Links

PubMed source DOI: 10.1128/jb.83.4.914-918.1962 PMC Full Text View journal details

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Purification of staphylococcal alpha-hemolysin.

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