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PMID: 1447288 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Restricted distribution of mRNA produced from a single nucleus in hybrid myotubes.

The Journal of cell biology ·Vol. 119 ·No. 5 ·1992-12-00 ·Pages 1063-8

Ralston E, Hall ZW

Abstract

Although the proteins encoded by a single nucleus in multinucleated myotubes have a wide range of distributions within the myofiber, little is known about the distributions of their mRNAs. We have used hybrid myotubes in which one or a few nuclei are derived from myoblasts that express nonmuscle proteins to investigate this question. We find that three different mRNAs, encoding proteins that are, respectively, nuclear, cytoplasmic, and targeted to the ER, have similar distributions within myotubes. Each is confined to an area within approximately 100 microns of the nucleus that expresses it.

MeSH Terms
Animals Biological Transport Cell Compartmentation Clone Cells Escherichia coli/enzymology Fluorescent Antibody Technique Hybrid Cells Mice Muscle Proteins/biosynthesis,isolation & purification Muscles/metabolism,ultrastructure Protein Sorting Signals/metabolism RNA, Messenger/isolation & purification Recombinant Fusion Proteins/isolation & purification,metabolism Transcription, Genetic Transfection beta-Galactosidase/isolation & purification,metabolism
Chemicals
Muscle Proteins Protein Sorting Signals RNA, Messenger Recombinant Fusion Proteins beta-Galactosidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ralston E
Department of Physiology, School of Medicine, University of California, San Francisco 94143-0444.
Hall Z W
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.119.5.1063
Published
1992-12-00
Pages
1063-8
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289719
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.119.5.1063 PMC Full Text View journal details
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