Incorporation of tritiated cytidine into ribonucleic acid by isolated pea nuclei.

The Journal of cell biology ·Vol. 14 ·1962-08-00 ·Pages 183-92

RHO JH, CHIPCHASE MI

Abstract

A new method for the isolation of the nuclei from plant tissue has been devised. This method consists in passing the plant tissue through a set of spring-loaded, counter-rotating rollers and collecting the liberated nuclei in sucrose solution containing calcium ions. A semi-automatic machine which couples the rollers with a special tissue chopping device has been constructed. It has been shown that nuclei obtained in this way actively incorporate cytidine-H(3) into RNA. This incorporation is increased in the presence of nucleoside triphosphates and an energy-regenerating system.

Keywords

NUCLEOSIDES AND NUCLEOTIDES/metabolism PLANTS/metabolism RIBONUCLEIC ACID/metabolism

MeSH Terms

Cell Nucleus Cytidine Nucleosides/metabolism Nucleotides/metabolism Peas Plants/metabolism RNA/metabolism

Chemicals

Nucleosides Nucleotides Cytidine RNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

RHO J H

CHIPCHASE M I

References (17)

17 references, click to expand

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Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.14.2.183

Published

1962-08-00

Pages

183-92

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2106106

Subset

External Links

PubMed source DOI: 10.1083/jcb.14.2.183 PMC Full Text View journal details

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Incorporation of tritiated cytidine into ribonucleic acid by isolated pea nuclei.

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