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PMID: 1450382 Published · ppublish English Journal Article

Ozone-induced changes of mRNA levels of beta-1,3-glucanase, chitinase and 'pathogenesis-related' protein 1b in tobacco plants.

Plant molecular biology ·Vol. 20 ·No. 4 ·1992-11-00 ·Pages 673-82

Ernst D, Schraudner M, Langebartels C, Sandermann H

Abstract

Treatment of the ozone-sensitive tobacco cultivar Bel W3 with an ozone pulse (0.15 microliter/l, 5 h) markedly increased the mRNA level of basic beta-1,3-glucanase and to a lower degree that of basic chitinase. The increase of beta-1,3-glucanase mRNA level occurred within 1 h and showed a transient maximum. Seventeen hours after ozone treatment, the beta-1,3-glucanase mRNA level decreased to lower values. The increase of basic chitinase mRNA level was delayed and was less pronounced than that of beta-1,3-glucanase mRNA. Cultivar Bel B showed only a small increase of beta-1,3-glucanase mRNA level after the same ozone treatment, whereas its basic chitinase mRNA was more strongly induced. Prolonged ozone treatment for 2 days of tobacco Bel W3 led to a persistent level of beta-1,3-glucanase and basic chitinase mRNAs, as well as to an increase of acidic chitinase and 'pathogenesis-related' (PR) 1b mRNA levels. The results indicate that genes so far considered to code for PR proteins may also be involved in the plant response to oxidative stress.

MeSH Terms
Blotting, Northern Chitinases/genetics,metabolism Gene Expression Regulation Glucan 1,3-beta-Glucosidase Kinetics Ozone/pharmacology Plant Proteins/genetics,metabolism Plants, Toxic RNA, Messenger/genetics Tobacco/enzymology,genetics beta-Glucosidase/genetics,metabolism
Chemicals
Plant Proteins RNA, Messenger pathogenesis-related proteins, plant Ozone Chitinases beta-Glucosidase Glucan 1,3-beta-Glucosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ernst D
GSF-Forschungszentrum für Umwelt und Gesundheit GmbH, Institut für Biochemische Pflanzenpathologie, Neuherberg, FRG.
Schraudner M
Langebartels C
Sandermann H
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00046452
Published
1992-11-00
Pages
673-82
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00046452 View journal details
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