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PMID: 1450385 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of an alanine aminotransferase from NAD-malic enzyme type C4 plant Panicum miliaceum.

Plant molecular biology ·Vol. 20 ·No. 4 ·1992-11-00 ·Pages 705-13

Son D, Sugiyama T

Abstract

We have determined the nucleotide sequence of a cDNA encoding AlaAT-2, which is believed to function in the C4-pathway of Panicum miliaceum. An open reading frame (1446 bp) encodes a protein of 482 amino acid residues. The deduced amino acid sequence of AlaAT-2 shows 44.2 and 44.8% homology with the amino acid sequences of AlaATs from rat and human livers, respectively. Northern blot analysis showed that the gene encoding AlaAT-2 in mesophyll and bundle sheath cells was the same and transcribed similarly in the cells. The level of translatable mRNA for AlaAT-2 increased dramatically during greening.

Related Genes
AlaAT
MeSH Terms
Alanine Transaminase/genetics,metabolism Amino Acid Sequence Animals Base Sequence Blotting, Southern Cloning, Molecular DNA/isolation & purification Humans Light Malate Dehydrogenase/genetics,metabolism Molecular Sequence Data Organ Specificity/genetics Panicum/enzymology,genetics,radiation effects Restriction Mapping Sequence Homology, Amino Acid
Chemicals
DNA Malate Dehydrogenase malate dehydrogenase-(oxaloacetate-decarboxylating) (NAD+) Alanine Transaminase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Son D
Department of Agricultural Chemistry, School of Agriculture, Nagoya University, Japan.
Sugiyama T
References (16)
16 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Selection of AUG initiation codons differs in plants and animals.
    EMBO J. 1987 Jan;6(1):43-8 PMID: 3556162
  3. Complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) determined by a combination of conventional and mass spectral methods.
    Biochemistry. 1991 Oct 29;30(43):10451-7 PMID: 1931970
  4. RNA extraction by the guanidine thiocyanate procedure.
    Methods Mol Biol. 1985;2:113-6 PMID: 21374179
  5. Immunoprecipitation of proteins from cell-free translations.
    Methods Enzymol. 1983;96:111-20 PMID: 6361451
  6. A simple high yield method for the preparation of lambda gt10 DNA suitable for subcloning, amplification and direct sequencing.
    Nucleic Acids Res. 1990 Jul 11;18(13):4031-2 PMID: 2142766
  7. Spatial regulation of photosynthetic development in C4 plants.
    Trends Genet. 1991 Jun;7(6):191-6 PMID: 1906211
  8. Cloning and sequence analysis of cDNA encoding aspartate aminotransferase isozymes from Panicum miliaceum L., a C4 plant.
    Eur J Biochem. 1992 Mar 1;204(2):611-20 PMID: 1541276
  9. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  10. Purification and characterization of alanine aminotransferase from Panicum miliaceum leaves.
    Arch Biochem Biophys. 1991 Sep;289(2):262-6 PMID: 1898070
  11. [On the binding of coenzyme to glutamic-pyruvic transaminase of pig heart].
    Eur J Biochem. 1968 Jun;5(1):18-23 PMID: 5660682
  12. Complete amino acid sequence of rat liver cytosolic alanine aminotransferase.
    Biochemistry. 1991 Jun 18;30(24):6048-53 PMID: 2043642
  13. Differential Expression in Bundle Sheath and Mesophyll Cells of Maize of Genes for Photosystem II Components Encoded by the Plastid Genome.
    Plant Physiol. 1988 Apr;86(4):1020-6 PMID: 16666025
  14. Separation of mesophyll protoplasts and bundle sheath cells from maize leaves for photosynthetic studies.
    Plant Physiol. 1973 Jun;51(6):1133-7 PMID: 16658479
  15. Pyridoxal 5'-phosphate binding site of pig heart alanine aminotransferase.
    Biochemistry. 1979 Jul 10;18(14):3002-7 PMID: 465450
  16. The purification of poly(a)-containing RNA by affinity chromatography.
    Methods Mol Biol. 1985;2:117-20 PMID: 21374180
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00046455
Published
1992-11-00
Pages
705-13
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00046455 View journal details
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