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PMID: 14517324 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The WD-repeats of Net2p interact with Dnm1p and Fis1p to regulate division of mitochondria.

Molecular biology of the cell ·Vol. 14 ·No. 10 ·2003-10-00 ·Pages 4126-39

Cerveny KL, Jensen RE

Abstract

The Net2, Fis1, and Dnm1 proteins are required for the division of mitochondria in the yeast Saccharomyces cerevisiae. Net2p has an amino-terminal region that contains predicted coiled-coil motifs and a carboxyl-terminal domain composed of WD-40 repeats. We found that the amino-terminal part of Net2p interacts with Fis1p, whereas the carboxyl-terminal region interacts with both Dnm1p and Fis1p. Overproduction of either domain of Net2p in yeast cells poisons mitochondrial fission, and the dominant-negative effect caused by the WD-repeats of Net2p is suppressed by increased levels of Dnm1p. Point mutations in the WD-region of Net2p or in the GTPase region of Dnm1p disrupt the normal Net2p-Dnm1p interaction, causing Net2p to lose its normal punctate distribution. Our results suggest that Dnm1p interacts with the WD-repeats of Net2p and in a GTP-dependent manner recruits Net2p to sites of mitochondrial division. Furthermore, our results indicate that Net2p is required for proper assembly of the mitochondrial fission components to regulate organelle division.

MeSH Terms
Adaptor Proteins, Signal Transducing Amino Acid Sequence Carrier Proteins/genetics,metabolism Cell Compartmentation/physiology GTP Phosphohydrolases/genetics,metabolism Microscopy, Fluorescence Mitochondria/metabolism,ultrastructure Mitochondrial Proteins/genetics,metabolism Molecular Sequence Data Plasmids/genetics Protein Binding/physiology Protein Structure, Tertiary/physiology Saccharomyces cerevisiae/cytology,genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Sequence Homology, Amino Acid Two-Hybrid System Techniques
Chemicals
Adaptor Proteins, Signal Transducing Carrier Proteins FIS1 protein, S cerevisiae MDV1 protein, S cerevisiae Mitochondrial Proteins Saccharomyces cerevisiae Proteins GTP Phosphohydrolases DNM1 protein, S cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cerveny Kara L
Department of Cell Biology, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Jensen Robert E
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e03-02-0092
Published
2003-10-00
Epub
2003-00-11
Pages
4126-39
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC207005
Subset
IM
Grants
NIGMS NIH HHS · R01 GM054021 · United States
NIGMS NIH HHS · R01-GM54021-06 · United States
External Links
PubMed source DOI: 10.1091/mbc.e03-02-0092 PMC Full Text View journal details
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