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PMID: 14521513 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S. Review

Isolation and characterization of functional mammary gland stem cells.

Cell proliferation ·Vol. 36 Suppl 1 ·2003-10-00 ·Pages 17-32

Welm B, Behbod F, Goodell MA, Rosen JM

Abstract

Significant advances in the stem-cell biology of several tissues, including the mammary gland, have occurred over the past several years. Recent progress on stem-cell fate determination, molecular markers, signalling pathways and niche interactions in haematopoietic, neuronal and muscle tissue may provide parallel insight into the biology of mammary epithelial stem cells. Taking advantage of approaches similar to those employed to isolate and characterize haematopoietic and epidermal stem cells, we have identified a mammary epithelial cell population with several stem/progenitor cell qualities. In this article, we review some recent data on mammary epithelial stem/progenitor cells in genetically engineered mouse models. We also discuss several potential molecular markers, including stem-cell antigen-1 (Sca-1), which may be useful for both the isolation of functional mammary epithelial stem/progenitor cells and the analysis of tumour aetiology and phenotype in genetically engineered mouse models. In different transgenic mammary tumour models, Sca-1 expression levels, as well as several other putative markers of progenitors including keratin-6, possess dramatically altered expression profiles. These data suggest that the heterogeneity of mouse models of breast cancer may partially reflect the selection or expansion of different progenitors.

MeSH Terms
Animals Cell Separation/methods Humans Mammary Glands, Animal/cytology Mammary Glands, Human/cytology Stem Cells/cytology,physiology
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Welm Bryan
Department of Anatomy, University of California, San Francisco, San Francisco, CA, USA.
Behbod Fariba
Goodell Margaret A
Rosen J M
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Article Info
Journal
Cell proliferation
Abbr.
Cell Prolif
ISSN
0960-7722
DOI
10.1046/j.1365-2184.36.s.1.3.x
Published
2003-10-00
Pages
17-32
Language
English
Region
England
NLM ID
9105195
PMCID
PMC3496772
Subset
IM
Grants
NCI NIH HHS · F32 CA101560 · United States
NCI NIH HHS · F32 CA101560-02 · United States
NCI NIH HHS · U01 CA084243 · United States
NCI NIH HHS · U01 CA84243 · United States
External Links
PubMed source DOI: 10.1046/j.1365-2184.36.s.1.3.x PMC Full Text View journal details
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