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PMID: 14526016 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A second PDZ-containing serine protease contributes to activation of the sporulation transcription factor sigmaK in Bacillus subtilis.

Journal of bacteriology ·Vol. 185 ·No. 20 ·2003-10-00 ·Pages 6051-6

Pan Q, Losick R, Rudner DZ

Abstract

Gene expression late during the process of sporulation in Bacillus subtilis is governed by a multistep, signal transduction pathway involving the transcription factor sigma(K), which is derived by regulated proteolysis from the inactive proprotein pro-sigma(K). Processing of pro-sigma(K) is triggered by a signaling protein known as SpoIVB, a serine protease that contains a region with similarity to the PDZ family of protein-protein interaction domains. Here we report the discovery of a second PDZ-containing serine protease called CtpB that contributes to the activation of the pro-sigma(K) processing pathway. CtpB is a sporulation-specific, carboxyl-terminal processing protease and shares several features with SpoIVB. We propose that CtpB acts to fine-tune the regulation of pro-sigma(K) processing, and we discuss possible models by which CtpB influences the sigma(K) activation pathway.

MeSH Terms
Bacillus subtilis/enzymology,genetics,physiology Bacterial Proteins/genetics,metabolism Gene Expression Regulation, Bacterial Protein Precursors/metabolism Serine Endopeptidases/chemistry,genetics,metabolism Signal Transduction Spores, Bacterial/physiology Time Factors Transcription Factors/metabolism Transcription, Genetic
Chemicals
Bacterial Proteins Protein Precursors Transcription Factors sigma K Serine Endopeptidases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pan Qi
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Losick Richard
Rudner David Z
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40 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.20.6051-6056.2003
Published
2003-10-00
Pages
6051-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC225033
Subset
IM
Grants
NIGMS NIH HHS · R01 GM018568 · United States
NIGMS NIH HHS · R37 GM018568 · United States
NIGMS NIH HHS · GM18568 · United States
External Links
PubMed source DOI: 10.1128/JB.185.20.6051-6056.2003 PMC Full Text View journal details
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