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PMID: 14551257 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The role of the polo kinase Cdc5 in controlling Cdc14 localization.

Molecular biology of the cell ·Vol. 14 ·No. 11 ·2003-11-00 ·Pages 4486-98

Visintin R, Stegmeier F, Amon A

Abstract

In budding yeast, the protein phosphatase Cdc14 controls exit from mitosis. Its activity is regulated by a competitive inhibitor Cfi1/Net1, which binds to and sequesters Cdc14 in the nucleolus. During anaphase, Cdc14 is released from its inhibitor by the action of two regulatory networks. The Cdc Fourteen Early Anaphase Release (FEAR) network initiates Cdc14 release from Cfi1/Net1 during early anaphase, and the Mitotic Exit Network (MEN) promotes Cdc14 release during late anaphase. Here, we investigate the relationship among FEAR network components and propose an order in which they function to promote Cdc14 release from the nucleolus. Furthermore, we examine the role of the protein kinase Cdc5, which is a component of both the FEAR network and the MEN, in Cdc14 release from the nucleolus. We find that overexpression of CDC5 led to Cdc14 release from the nucleolus in S phase-arrested cells, which correlated with the appearance of phosphorylated forms of Cdc14 and Cfi1/Net1. Cdc5 promotes Cdc14 phosphorylation and, by stimulating the MEN, Cfi1/Net1 phosphorylation. Furthermore, we suggest that Cdc14 release from the nucleolus only occurs when Cdc14 and Cfi1/Net1 are both phosphorylated.

MeSH Terms
Cell Cycle Proteins/metabolism Cell Nucleolus/enzymology Fungal Proteins/metabolism Microscopy, Fluorescence Mitosis Models, Molecular Mutation Nuclear Proteins/metabolism Phosphorylation Protein Kinases/metabolism Protein Serine-Threonine Kinases Protein Tyrosine Phosphatases/metabolism Saccharomyces cerevisiae/enzymology Saccharomyces cerevisiae Proteins/metabolism Signal Transduction
Chemicals
CDC14 protein, S cerevisiae Cell Cycle Proteins Fungal Proteins Net1 protein, S cerevisiae Nuclear Proteins SPO12 protein, S cerevisiae Saccharomyces cerevisiae Proteins Protein Kinases Protein Serine-Threonine Kinases CDC5 protein, S cerevisiae Protein Tyrosine Phosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Visintin Rosella
Center for Cancer Research, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Stegmeier Frank
Amon Angelika
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e03-02-0095
Published
2003-11-00
Epub
2003-00-07
Pages
4486-98
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC266767
Subset
IM
Grants
NIGMS NIH HHS · R01 GM056800 · United States
NIGMS NIH HHS · R29 GM056800 · United States
NIGMS NIH HHS · GM-56800 · United States
External Links
PubMed source DOI: 10.1091/mbc.e03-02-0095 PMC Full Text View journal details
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