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PMID: 14555692 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Proanthocyanidin-accumulating cells in Arabidopsis testa: regulation of differentiation and role in seed development.

The Plant cell ·Vol. 15 ·No. 11 ·2003-11-00 ·Pages 2514-31

Debeaujon I, Nesi N, Perez P, Devic M, Grandjean O, Caboche M, Lepiniec L

Abstract

Anthocyanidin reductase encoded by the BANYULS (BAN) gene is the core enzyme in proanthocyanidin (PA) biosynthesis. Here, we analyzed the developmental mechanisms that regulate the spatiotemporal expression of BAN in the developing Arabidopsis seed coat. PA-accumulating cells were localized histochemically in the inner integument (seed body and micropyle) and pigment strand (chalaza). BAN promoter activity was detected specifically in these cells. Gain-of-function experiments showed that an 86-bp promoter fragment functioned as an enhancer specific for PA-accumulating cells. Mutations in regulatory genes of PA biosynthesis abolished BAN promoter activity (transparent testa2 [tt2], tt8, and transparent testa glabra1 [ttg1]), modified its spatial pattern (tt1 and tt16), or had no influence (ttg2), thus revealing complex regulatory interactions at several developmental levels. Genetic ablation of PA-accumulating cells targeted by the BAN promoter fused to BARNASE led to the formation of normal plants that produced viable yellow seeds. Importantly, these seeds had no obvious defects in endosperm and embryo development.

MeSH Terms
Arabidopsis/genetics,growth & development,metabolism Arabidopsis Proteins/genetics,metabolism Base Sequence Cell Differentiation/genetics,physiology DNA-Binding Proteins/genetics,metabolism Gene Expression Regulation, Developmental Gene Expression Regulation, Plant MADS Domain Proteins/genetics,metabolism Molecular Sequence Data Mutation NADH, NADPH Oxidoreductases/genetics,metabolism Proanthocyanidins/biosynthesis,metabolism Promoter Regions, Genetic/genetics Seeds/genetics,growth & development,metabolism Sequence Homology, Nucleic Acid Tannins/biosynthesis
Chemicals
Arabidopsis Proteins DNA-Binding Proteins MADS Domain Proteins Proanthocyanidins TT16 protein, Arabidopsis TT2 protein, Arabidopsis Tannins BANYULS protein, Arabidopsis NADH, NADPH Oxidoreductases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Debeaujon Isabelle
Laboratoire de Biologie des Semences, Unité Mixte de Recherche, 204 Institut National de la Recherche Agronomique, Paris-Grignon, 78026 Versailles, France.
Nesi Nathalie
Perez Pascual
Devic Martine
Grandjean Olivier
Caboche Michel
Lepiniec Loïc
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.014043
Published
2003-11-00
Epub
2003-00-10
Pages
2514-31
Language
English
Region
England
NLM ID
9208688
PMCID
PMC280558
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.014043 PMC Full Text View journal details
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