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PMID: 14560021 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dynamic properties of nucleosomes during thermal and ATP-driven mobilization.

Molecular and cellular biology ·Vol. 23 ·No. 21 ·2003-11-00 ·Pages 7767-79

Flaus A, Owen-Hughes T

Abstract

The fundamental subunit of chromatin, the nucleosome, is not a static entity but can move along DNA via either thermal or enzyme-driven movements. Here we have monitored the movements of nucleosomes following deposition at well-defined locations on mouse mammary tumor virus promoter DNA. We found that the sites to which nucleosomes are deposited during chromatin assembly differ from those favored during thermal equilibration. Taking advantage of this, we were able to track the movement of nucleosomes over 156 bp and found that this proceeds via intermediate positions spaced between 46 and 62 bp. The remodeling enzyme ISWI was found to direct the movement of nucleosomes to sites related to those observed during thermal mobilization. In contrast, nucleosome mobilization driven by the SWI/SNF and RSC complexes were found to drive nucleosomes towards sites up to 51 bp beyond DNA ends, with little respect for the sites favored during thermal repositioning. The dynamic properties of nucleosomes we describe are likely to influence their role in gene regulation.

MeSH Terms
Adenosine Triphosphatases/metabolism Adenosine Triphosphate/metabolism Animals DNA/metabolism Histones/metabolism Macromolecular Substances Mammary Tumor Virus, Mouse/genetics Mice Nucleosomes/metabolism Promoter Regions, Genetic Temperature Transcription Factors/metabolism
Chemicals
Histones ISWI protein Macromolecular Substances Nucleosomes Transcription Factors Adenosine Triphosphate DNA Adenosine Triphosphatases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Flaus Andrew
Division of Gene Regulation and Expression, The Wellcome Trust Biocentre, University of Dundee, Dundee DD1 5EH, United Kingdom.
Owen-Hughes Tom
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.23.21.7767-7779.2003
Published
2003-11-00
Pages
7767-79
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC207611
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.23.21.7767-7779.2003 PMC Full Text View journal details
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