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PMID: 1459406 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Temperature-sensitive mutants of the Mycobacterium plasmid pAL5000.

FEMS microbiology letters ·Vol. 77 ·No. 1-3 ·1992-11-01 ·Pages 181-6

Guilhot C, Gicquel B, Martín C

Abstract

Two plasmids were isolated as thermosensitive replicons following in vitro mutagenesis of pB4, a pAL5000 derivative mycobacteria/Escherichia coli shuttle plasmid. Plasmids pCG59 and pCG63 replicate at 30 degrees C but not at 39 degrees C. This will allow their utilisation for transposon delivery, site-specific integration, or allele exchange.

MeSH Terms
Escherichia coli/genetics Genes, Bacterial Genetic Vectors Mutagenesis Mycobacterium/genetics Plasmids Replicon Temperature Transformation, Genetic
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Guilhot C
Unité de Génie Microbiologique, URA1300 du CNRS, Institut Pasteur, Paris, France.
Gicquel B
Martín C
Article Info
Journal
FEMS microbiology letters
Abbr.
FEMS Microbiol Lett
ISSN
0378-1097
DOI
10.1016/0378-1097(92)90152-e
Published
1992-11-01
Pages
181-6
Language
English
Region
England
NLM ID
7705721
Subset
IM
External Links
PubMed source DOI: 10.1016/0378-1097(92)90152-e View journal details
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