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PMID: 14602640 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Binding of pediocin PA-1 with anionic lipid induces model membrane destabilization.

Applied and environmental microbiology ·Vol. 69 ·No. 11 ·2003-11-00 ·Pages 6777-84

Gaussier H, Lefèvre T, Subirade M

Abstract

To obtain molecular insights into the action mode of antimicrobial activity of pediocin PA-1, the interactions between this bacteriocin and dimyristoylphosphatidylcholine (DMPC) or dimyristoylphosphatidylglycerol (DMPG) model membranes have been investigated in D(2)O at pD 6 by Fourier transform infrared spectroscopy. The interactions were monitored with respect to alteration of the secondary structure of pediocin, as registered by the amide I' band, and phospholipid conformation, as revealed by the methylene nu(s)(CH(2)) and carbonyl nu(C;O) stretching vibrations. The results show that no interaction between pediocin and DMPC occurs. By contrast, pediocin undergoes a structural reorganization in the presence of DMPG. Upon heating, pediocin self-aggregates, which is not observed for this pD in aqueous solution. The gel-to-crystalline phase transition of DMPG shifts to higher temperatures with a concomitant dehydration of the interfacial region. Our results indicate that pediocin is an extrinsic peptide and that its action mechanism may lie in a destabilization of the cell membrane.

MeSH Terms
Anti-Bacterial Agents/chemistry,metabolism Bacteriocins/chemistry,metabolism Cell Membrane/chemistry,metabolism Dimyristoylphosphatidylcholine/metabolism Lipid Bilayers/chemistry,metabolism Membrane Lipids/metabolism Models, Biological Pediocins Phosphatidylglycerols/metabolism Protein Conformation Spectroscopy, Fourier Transform Infrared
Chemicals
Anti-Bacterial Agents Bacteriocins Lipid Bilayers Membrane Lipids Pediocins Phosphatidylglycerols pediocin PA-1 dimyristoylphosphatidylglycerol Dimyristoylphosphatidylcholine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gaussier Hélène
Centre de recherche en Sciences et Technologie du Lait (STELA), and Institut sur les nutraceutiques et aliments fonctionnels (INAF), Département des sciences des aliments et de nutrition, Université Laval, Pavillon Paul-Comtois, Sainte-Foy, Québec, Canada.
Lefèvre Thierry
Subirade Muriel
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/AEM.69.11.6777-6784.2003
Published
2003-11-00
Pages
6777-84
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC262285
Subset
IM
External Links
PubMed source DOI: 10.1128/AEM.69.11.6777-6784.2003 PMC Full Text View journal details
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