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PMID: 14605235 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Activation of anthocyanin biosynthesis in Gerbera hybrida (Asteraceae) suggests conserved protein-protein and protein-promoter interactions between the anciently diverged monocots and eudicots.

Plant physiology ·Vol. 133 ·No. 4 ·2003-12-00 ·Pages 1831-42

Elomaa P, Uimari A, Mehto M, Albert VA, Laitinen RA, Teeri TH

Abstract

We have identified an R2R3-type MYB factor, GMYB10, from Gerbera hybrida (Asteraceae) that shares high sequence homology to and is phylogenetically grouped together with the previously characterized regulators of anthocyanin pigmentation in petunia (Petunia hybrida) and Arabidopsis. GMYB10 is able to induce anthocyanin pigmentation in transgenic tobacco (Nicotiana tabacum), especially in vegetative parts and anthers. In G. hybrida, GMYB10 is involved in activation of anthocyanin biosynthesis in leaves, floral stems, and flowers. In flowers, its expression is restricted to petal epidermal cell layers in correlation with the anthocyanin accumulation pattern. We have shown, using yeast (Saccharomyces cerevisiae) two-hybrid assay, that GMYB10 interacts with the previously isolated bHLH factor GMYC1. Particle bombardment analysis was used to show that GMYB10 is required for activation of a late anthocyanin biosynthetic gene promoter, PGDFR2. cis-Analysis of the target PGDFR2 revealed a sequence element with a key role in activation by GMYB10/GMYC1. This element shares high homology with the anthocyanin regulatory elements characterized in maize (Zea mays) anthocyanin promoters, suggesting that the regulatory mechanisms involved in activation of anthocyanin biosynthesis have been conserved for over 125 million years not only at the level of transcriptional regulators but also at the level of the biosynthetic gene promoters.

MeSH Terms
Amino Acid Sequence Anthocyanins/biosynthesis Asteraceae/classification,genetics,metabolism Base Sequence Cloning, Molecular Conserved Sequence Cotyledon/genetics,metabolism Molecular Sequence Data Phylogeny Plant Proteins/genetics,metabolism Promoter Regions, Genetic/genetics Saccharomyces cerevisiae/genetics Sequence Alignment Sequence Homology, Amino Acid Transcription Factors/genetics,metabolism
Chemicals
Anthocyanins Plant Proteins Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Elomaa Paula
Department of Applied Biology, PO Box 27, University of Helsinki, Helsinki FIN-00014, Finland. [email protected]
Uimari Anne
Mehto Merja
Albert Victor A
Laitinen Roosa A E
Teeri Teemu H
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.103.026039
Email
[email protected]
Published
2003-12-00
Epub
2003-00-06
Pages
1831-42
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC300736
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.103.026039 PMC Full Text View journal details
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