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PMID: 14606955 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Production and characterization of reduced NAADP (nicotinic acid-adenine dinucleotide phosphate).

The Biochemical journal ·Vol. 378 ·No. Pt 1 ·2004-02-15 ·Pages 275-80

Billington RA, Thuring JW, Conway SJ, Packman L, Holmes AB, Genazzani AA

Abstract

The pyridine nucleotide NAADP (nicotinic acid-adenine dinucleotide phosphate) has been shown to act as a Ca2+-releasing intracellular messenger in a wide variety of systems from invertebrates to mammals and has been implicated in a number of cellular processes. NAADP is structurally very similar to its precursor, the endogenous coenzyme NADP and while much is known about the reduced form of NADP, NADPH, it is not known whether NAADP can also exist in a reduced state. Here we report that NAADP can be reduced to NAADPH by endogenous cellular enzymes and that NAADPH is functionally inert at the NAADP receptor. These data suggest that NAADPH could represent a mechanism for rapidly inactivating NAADP in cells.

MeSH Terms
Animals Calcium/metabolism Carrier Proteins/metabolism Glucosephosphate Dehydrogenase/metabolism NADP/analogs & derivatives,chemistry,metabolism,pharmacology Ovum/metabolism Oxidation-Reduction Sea Urchins/embryology
Chemicals
Carrier Proteins NADP NAADP Glucosephosphate Dehydrogenase Calcium
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Billington Richard A
Department of Pharmacology, Tennis Court Road, University of Cambridge, Cambridge CB2 1PD, UK.
Thuring Jan W
Conway Stuart J
Packman Len
Holmes Andrew B
Genazzani Armando A
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
1470-8728
DOI
10.1042/BJ20031284
Published
2004-02-15
Pages
275-80
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1223936
Subset
IM
External Links
PubMed source DOI: 10.1042/BJ20031284 PMC Full Text View journal details
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