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PMID: 1463009 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Analysis of chromosome 21 yeast artificial chromosome (YAC) clones.

American journal of human genetics ·Vol. 51 ·No. 6 ·1992-12-00 ·Pages 1251-64

Tassone F, Cheng S, Gardiner K

Abstract

Chromosome 21 contains genes relevant to several important diseases. Yeast artificial chromosome (YAC) clones, because they span > 100 kbp, will provide attractive material for initiating searches for such genes. Twenty-two YAC clones, each of which maps to a region of potential relevance either to aspects of the Down syndrome phenotype or to one of the other chromosome 21-associated genetic diseases, have been analyzed in detail. Clones total approximately 6,000 kb and derive from all parts of the long arm. Rare restriction-site maps have been constructed for each clone and have been used to determine regional variations in clonability, methylation frequency, CpG island density, and CpG island frequency versus gene density. This information will be useful for the isolation and mapping of new genes to chromosome 21 and for walking in YAC libraries.

MeSH Terms
Chromosome Mapping Chromosomes, Fungal Chromosomes, Human, Pair 21 Dinucleoside Phosphates/analysis Electrophoresis, Gel, Pulsed-Field Gene Frequency Genome, Human Genomic Library Humans Methylation Multigene Family Restriction Mapping
Chemicals
Dinucleoside Phosphates cytidylyl-3'-5'-guanosine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tassone F
Eleanor Roosevelt Institute for Cancer Research, Denver, CO 80206.
Cheng S
Gardiner K
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Article Info
Journal
American journal of human genetics
Abbr.
Am J Hum Genet
ISSN
0002-9297
Published
1992-12-00
Pages
1251-64
Language
English
Region
United States
NLM ID
0370475
PMCID
PMC1682922
Subset
IM
Grants
NICHD NIH HHS · HD17449 · United States
NHGRI NIH HHS · HG00001 · United States
External Links
PubMed source PMC Full Text View journal details
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