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PMID: 14632665 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Induction of protective immunity against a Chlamydia trachomatis genital infection in three genetically distinct strains of mice.

Immunology ·Vol. 110 ·No. 3 ·2003-11-00 ·Pages 368-75

Pal S, Peterson EM, de la Maza LM

Abstract

To establish the feasibility of inducing a protective immune response against a chlamydial genital infection in animals with different genetic backgrounds, groups of C3H/HeN (H-2k), BALB/c (H-2d) and C57BL/6 (H-2b) mice, were immunized intranasally with elementary bodies (EB) of the Chlamydia trachomatis mouse pneumonitis biovar. Following the intranasal immunization strong Chlamydia-specific humoral and cell-mediated immune (CMI) responses were detected in the three strains of mice. Eight weeks following immunization the animals were challenged with C. trachomatis in the genital tract. Vaginal cultures showed that the three strains of mice immunized with EB were significantly protected in comparison to the sham immunized animals. To determine the ability of this immunization protocol to protect against infertility six weeks after the genital challenge the animals were mated. Mice of the three strains immunized with EB showed significant protection as demonstrated by the number of animals that were fertile, and the number of embryos present in their uterine horns, in comparison to the sham immunized mice.

MeSH Terms
Animals Antibodies, Bacterial/biosynthesis Bacterial Vaccines/immunology Cells, Cultured Chlamydia Infections/complications,immunology,prevention & control Chlamydia trachomatis/immunology Female Genetic Predisposition to Disease Genital Diseases, Female/immunology,prevention & control Infertility, Female/immunology,microbiology,prevention & control Mice Mice, Inbred BALB C Mice, Inbred C57BL Species Specificity Spleen/immunology Vagina/immunology,microbiology
Chemicals
Antibodies, Bacterial Bacterial Vaccines
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pal Sukumar
Department of Pathology, University of California, Irvine, Irvine, CA 92697-4800, USA.
Peterson Ellena M
de la Maza Luis M
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
DOI
10.1046/j.1365-2567.2003.01748.x
Published
2003-11-00
Pages
368-75
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1783049
Subset
IM
Grants
NIAID NIH HHS · R01 AI032248 · United States
NIAID NIH HHS · AI-32248 · United States
External Links
PubMed source DOI: 10.1046/j.1365-2567.2003.01748.x PMC Full Text View journal details
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