What's cooking? detection of important biomarkers in HOPE-fixed, paraffin-embedded tissues eliminates the need for antigen retrieval.

The American journal of pathology ·Vol. 163 ·No. 6 ·2003-12-00 ·Pages 2638-40

Goldmann T, Vollmer E, Gerdes J

Abstract

暂无摘要

MeSH Terms

Antigens/isolation & purification Biomarkers/analysis Fixatives Humans Immunohistochemistry/methods Paraffin Embedding Tissue Fixation/methods

Chemicals

Antigens Biomarkers Fixatives

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Goldmann Torsten

Vollmer Ekkehard

Gerdes Johannes

References (10)

10 references, click to expand

Immunohistological detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues.
J Pathol. 1992 Sep;168(1):85-6 PMID: 1453271
Quantitative gene expression analysis in microdissected archival formalin-fixed and paraffin-embedded tumor tissue.
Am J Pathol. 2001 Feb;158(2):419-29 PMID: 11159180
Evaluating HER2 amplification and overexpression in breast cancer.
J Pathol. 2001 Nov;195(4):422-8 PMID: 11745673
The expression of Ki-67, MCM3, and p27 defines distinct subsets of proliferating, resting, and differentiated cells.
J Pathol. 2001 Nov;195(4):457-62 PMID: 11745678
Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation.
Int J Cancer. 1983 Jan 15;31(1):13-20 PMID: 6339421
Assessment of transcriptional gene activity in situ by application of HOPE-fixed, paraffin-embedded tissues.
Pathol Res Pract. 2002;198(2):91-5 PMID: 11928870
Quality assurance in immunohistochemistry: results of an interlaboratory trial involving 172 pathologists.
Am J Surg Pathol. 2002 Jul;26(7):873-82 PMID: 12131154
Inter-laboratory and inter-observer reproducibility of immunohistochemical assessment of the Ki-67 labelling index in a large multi-centre trial.
J Pathol. 2002 Nov;198(3):292-9 PMID: 12375261
HOPE--a new fixing technique enables preservation and extraction of high molecular weight DNA and RNA of > 20 kb from paraffin-embedded tissues. Hepes-Glutamic acid buffer mediated Organic solvent Protection Effect.
Pathol Res Pract. 2002;198(11):735-40 PMID: 12530576
HOPE fixation: a novel fixing method and paraffin-embedding technique for human soft tissues.
Pathol Res Pract. 2001;197(12):823-6 PMID: 11795830

Article Info

Journal

The American journal of pathology

Abbr.

Am J Pathol

ISSN

0002-9440

DOI

10.1016/S0002-9440(10)63619-X

Published

2003-12-00

Pages

2638-40

Language

English

Region

United States

NLM ID

0370502

PMCID

PMC1892364

Subset

External Links

PubMed source DOI: 10.1016/S0002-9440(10)63619-X PMC Full Text View journal details

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What's cooking? detection of important biomarkers in HOPE-fixed, paraffin-embedded tissues eliminates the need for antigen retrieval.

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