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PMID: 14657018 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Ribosome binding to the Oxa1 complex facilitates co-translational protein insertion in mitochondria.

The EMBO journal ·Vol. 22 ·No. 24 ·2003-12-15 ·Pages 6448-57

Szyrach G, Ott M, Bonnefoy N, Neupert W, Herrmann JM

Abstract

The Oxa1 translocase of the mitochondrial inner membrane facilitates the insertion of both mitochondrially and nuclear-encoded proteins from the matrix into the inner membrane. Most mitochondrially encoded proteins are hydrophobic membrane proteins which are integrated into the lipid bilayer during their synthesis on mitochondrial ribosomes. The molecular mechanism of this co-translational insertion process is unknown. Here we show that the matrix-exposed C-terminus of Oxa1 forms an alpha-helical domain that has the ability to bind to mitochondrial ribosomes. Deletion of this Oxa1 domain strongly diminished the efficiency of membrane insertion of subunit 2 of cytochrome oxidase, a mitochondrially encoded substrate of the Oxa1 translocase. This suggests that co-translational membrane insertion of mitochondrial translation products is facilitated by a physical interaction of translation complexes with the membrane-bound translocase.

MeSH Terms
Chromatography, Affinity Electron Transport Complex IV Glutathione Transferase/metabolism Intracellular Membranes/physiology Mitochondria/physiology Mitochondrial Proteins Nuclear Proteins/genetics,metabolism Oxygen Consumption Protein Biosynthesis Protein Transport Recombinant Fusion Proteins/isolation & purification,metabolism Ribosomal Proteins/isolation & purification,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism
Chemicals
Mitochondrial Proteins Nuclear Proteins OXA1 protein Recombinant Fusion Proteins Ribosomal Proteins Saccharomyces cerevisiae Proteins Electron Transport Complex IV Glutathione Transferase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Szyrach Gregor
Institut für Physiologische Chemie, Butenandtstrasse 5, D-81377 München, Germany.
Ott Martin
Bonnefoy Nathalie
Neupert Walter
Herrmann Johannes M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/cdg623
Published
2003-12-15
Pages
6448-57
Language
English
Region
England
NLM ID
8208664
PMCID
PMC291818
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/cdg623 PMC Full Text View journal details
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