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PMID: 14718521 Published · ppublish English Journal Article

Essential role of MARCKS in cortical actin dynamics during gastrulation movements.

The Journal of cell biology ·Vol. 164 ·No. 2 ·2004-01-19 ·Pages 169-74

Iioka H, Ueno N, Kinoshita N

Abstract

Myristoylated alanine-rich C kinase substrate (MARCKS) is an actin-binding, membrane-associated protein expressed during Xenopus embryogenesis. We analyzed its function in cytoskeletal regulation during gastrulation. Here, we show that blockade of its function impaired morphogenetic movements, including convergent extension. MARCKS was required for control of cell morphology, motility, adhesion, protrusive activity, and cortical actin formation in embryonic cells. We also demonstrate that the noncanonical Wnt pathway promotes the formation of lamellipodia- and filopodia-like protrusions and that MARCKS is necessary for this activity. These findings show that MARCKS regulates the cortical actin formation that is requisite for dynamic morphogenetic movements.

MeSH Terms
Actins/physiology Animals Cell Movement/physiology Cloning, Molecular Embryo, Nonmammalian/physiology Gastrula/physiology In Situ Hybridization Intracellular Signaling Peptides and Proteins Lipoproteins/genetics,physiology Membrane Proteins/genetics,physiology Myristoylated Alanine-Rich C Kinase Substrate Protein Kinase C/physiology RNA, Messenger/genetics Reverse Transcriptase Polymerase Chain Reaction Xenopus/embryology
Chemicals
Actins Intracellular Signaling Peptides and Proteins Lipoproteins Membrane Proteins RNA, Messenger Myristoylated Alanine-Rich C Kinase Substrate Protein Kinase C
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Iioka Hidekazu
Dept. of Developmental Biology, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji Okazaki, Aichi 444-8585, Japan.
Ueno Naoto
Kinoshita Noriyuki
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200310027
Published
2004-01-19
Epub
2004-00-12
Pages
169-74
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2172330
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.200310027 PMC Full Text View journal details
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