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PMID: 14729917 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

MASSUGU2 encodes Aux/IAA19, an auxin-regulated protein that functions together with the transcriptional activator NPH4/ARF7 to regulate differential growth responses of hypocotyl and formation of lateral roots in Arabidopsis thaliana.

The Plant cell ·Vol. 16 ·No. 2 ·2004-02-00 ·Pages 379-93

Tatematsu K, Kumagai S, Muto H, Sato A, Watahiki MK, Harper RM, Liscum E, Yamamoto KT

Abstract

We have isolated a dominant, auxin-insensitive mutant of Arabidopsis thaliana, massugu2 (msg2), that displays neither hypocotyl gravitropism nor phototropism, fails to maintain an apical hook as an etiolated seedling, and is defective in lateral root formation. Yet other aspects of growth and development of msg2 plants are almost normal. These characteristics of msg2 are similar to those of another auxin-insensitive mutant, non-phototropic hypocotyl4 (nph4), which is a loss-of-function mutant of AUXIN RESPONSE FACTOR7 (ARF7) (Harper et al., 2000). Map-based cloning of the MSG2 locus reveals that all four mutant alleles result in amino acid substitutions in the conserved domain II of an Auxin/Indole-3-Acetic Acid protein, IAA19. Interestingly, auxin inducibility of MSG2/IAA19 gene expression is reduced by 65% in nph4/arf7. Moreover, MSG2/IAA19 protein binds to the C-terminal domain of NPH4/ARF7 in a Saccharomyces cerevisiae (yeast) two-hybrid assay and to the whole latter protein in vitro by pull-down assay. These results suggest that MSG2/IAA19 and NPH4/ARF7 may constitute a negative feedback loop to regulate differential growth responses of hypocotyls and lateral root formation.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,growth & development,metabolism Arabidopsis Proteins/genetics,metabolism Chlorophyll/metabolism Gene Expression Regulation, Developmental/drug effects Gene Expression Regulation, Plant/drug effects Hypocotyl/drug effects,genetics,growth & development,metabolism Indoleacetic Acids/pharmacology Molecular Sequence Data Mutation Nuclear Proteins/genetics,metabolism Phenotype Plant Growth Regulators/genetics,metabolism,pharmacology Plant Roots/genetics,growth & development,metabolism Reproduction/genetics,physiology Saccharomyces cerevisiae/genetics Trans-Activators/genetics,metabolism Two-Hybrid System Techniques
Chemicals
Arabidopsis Proteins DFL1 protein, Arabidopsis IAA19 protein, Arabidopsis IAA4 protein, Arabidopsis Indoleacetic Acids Nuclear Proteins Plant Growth Regulators SAUR-AC1 protein, Arabidopsis Trans-Activators Chlorophyll
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Tatematsu Kiyoshi
Division of Biological Sciences, Graduate School of Environmental Earth Science, Hokkaido University, Sapporo 060-0810, Japan.
Kumagai Satoshi
Muto Hideki
Sato Atsuko
Watahiki Masaaki K
Harper Reneé M
Liscum Emmanuel
Yamamoto Kotaro T
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.018630
Published
2004-02-00
Epub
2004-00-16
Pages
379-93
Language
English
Region
England
NLM ID
9208688
PMCID
PMC341911
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.018630 PMC Full Text View journal details
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