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PMID: 14730075 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel family of transporters mediating the transport of glutathione derivatives in plants.

Plant physiology ·Vol. 134 ·No. 1 ·2004-01-00 ·Pages 482-91

Zhang MY, Bourbouloux A, Cagnac O, Srikanth CV, Rentsch D, Bachhawat AK, Delrot S

Abstract

Uptake and compartmentation of reduced glutathione (GSH), oxidized glutathione (GSSG), and glutathione conjugates are important for many functions including sulfur transport, resistance against biotic and abiotic stresses, and developmental processes. Complementation of a yeast (Saccharomyces cerevisiae) mutant (hgt1) deficient in glutathione transport was used to characterize a glutathione transporter cDNA (OsGT1) from rice (Oryza sativa). The 2.58-kb full-length cDNA (AF393848, gi 27497095), which was obtained by screening of a cDNA library and 5'-rapid amplification of cDNA ends-polymerase chain reaction, contains an open reading frame encoding a 766-amino acid protein. Complementation of the hgt1 yeast mutant strain with the OsGT1 cDNA restored growth on a medium containing GSH as the sole sulfur source. The strain expressing OsGT1 mediated [3H]GSH uptake, and this uptake was significantly competed not only by unlabeled GSSG and GS conjugates but also by some amino acids and peptides, suggesting a wide substrate specificity. OsGT1 may be involved in the retrieval of GSSG, GS conjugates, and nitrogen-containing peptides from the cell wall.

MeSH Terms
Carrier Proteins/genetics,metabolism DNA, Complementary/genetics,isolation & purification DNA, Plant/genetics,isolation & purification Energy Metabolism Genetic Complementation Test Genome, Bacterial Glutathione/metabolism Membrane Transport Proteins Mutation Oryza/genetics,metabolism Phylogeny Plant Proteins/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism
Chemicals
Carrier Proteins DNA, Complementary DNA, Plant Membrane Transport Proteins Plant Proteins glutathione transporter Glutathione
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Zhang Ming-Yong
Unité Mixte de Recherche Centre National de la Recherche Scientifique 6161, Transport des Assimilats, Laboratoire de Physiologie, Biochimie et Biologie Moléculaires Végétales, Unité de Formation et de Recherche Sciences, Poitiers, France.
Bourbouloux Andrée
Cagnac Olivier
Srikanth Chittur V
Rentsch Doris
Bachhawat Anand K
Delrot Serge
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.103.030940
Published
2004-01-00
Pages
482-91
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC316327
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.103.030940 PMC Full Text View journal details
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