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PMID: 14742263 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Basal cells are a multipotent progenitor capable of renewing the bronchial epithelium.

The American journal of pathology ·Vol. 164 ·No. 2 ·2004-02-00 ·Pages 577-88

Hong KU, Reynolds SD, Watkins S, Fuchs E, Stripp BR

Abstract

Commitment of the pulmonary epithelium to bronchial and bronchiolar airway lineages occurs during the transition from pseudoglandular to cannalicular phases of lung development, suggesting that regional differences exist with respect to the identity of stem and progenitor cells that contribute to epithelial maintenance in adulthood. We previously defined a critical role for Clara cell secretory protein-expressing (CE) cells in renewal of bronchiolar airway epithelium following injury. Even though CE cells are also the principal progenitor for maintenance of the bronchial airway epithelium, CE cell injury is resolved through a mechanism involving recruitment of a second progenitor cell population that we now identify as a GSI-B(4) reactive, cytokeratin-14-expressing basal cell. These cells exhibit multipotent differentiation capacity as assessed by analysis of cellular phenotype within clones of LacZ-tagged cells. Clones were derived from K14-expressing cells tagged in a cell-type-specific fashion by ligand-regulable Cre recombinase-mediated genomic rearrangement of the ROSA26 recombination substrate allele. We conclude that basal cells represent an alternative multipotent progenitor cell population of bronchial airways and that progenitor cell selection is dictated by the type of airway injury.

MeSH Terms
Animals Antiviral Agents/pharmacology Bronchi/cytology,drug effects,physiology Cell Lineage Epithelium/drug effects,physiology Estrogen Antagonists/pharmacology Female Ganciclovir/pharmacology Immunohistochemistry Keratins/metabolism Mice Mice, Transgenic Naphthalenes/pharmacology Regeneration/physiology Stem Cells/cytology,drug effects Tamoxifen/pharmacology
Chemicals
Antiviral Agents Estrogen Antagonists Naphthalenes Tamoxifen naphthalene Keratins Ganciclovir
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hong Kyung U
Department of Environmental Medicine, University of Rochester, Rochester, New York, USA.
Reynolds Susan D
Watkins Simon
Fuchs Elaine
Stripp Barry R
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
DOI
10.1016/S0002-9440(10)63147-1
Published
2004-02-00
Pages
577-88
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1602270
Subset
IM
Grants
NIEHS NIH HHS · R01 ES008964 · United States
NHLBI NIH HHS · R01 HL064888 · United States
NIEHS NIH HHS · ES08964 · United States
NHLBI NIH HHS · HL64888 · United States
External Links
PubMed source DOI: 10.1016/S0002-9440(10)63147-1 PMC Full Text View journal details
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