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PMID: 14747652 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Long-term effects of culture of preimplantation mouse embryos on behavior.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 101 ·No. 6 ·2004-02-10 ·Pages 1595-600

Ecker DJ, Stein P, Xu Z, Williams CJ, Kopf GS, Bilker WB, Abel T, Schultz RM

Abstract

Many procedures used in assisted reproductive technologies (ART) to treat human infertility entail culture of preimplantation embryos. Moreover, there is an increasing trend to culture embryos for longer periods of time before uterine transfer to identify the "best" embryos for transfer and to minimize multiple pregnancies. Embryo culture, however, can perturb embryo metabolism and gene expression, and the long-term consequences of culture are unknown. We have explored the behavioral consequences of embryo culture by using a 129S6/SvEvTac/C57BL/6J F(1) mouse model and find that adults derived from cultured embryos exhibit specific behavioral alterations in the elevated zero maze and Morris water maze tasks.

MeSH Terms
Animals Anxiety Behavior, Animal Embryonic Development Female Maze Learning Mice Pregnancy
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ecker David J
Department of Biology, University of Pennsylvania, 415 South University Avenue, Philadelphia, PA 19104, USA.
Stein Paula
Xu Zhe
Williams Carmen J
Kopf Gregory S
Bilker Warren B
Abel Ted
Schultz Richard M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0306846101
Published
2004-02-10
Epub
2004-00-27
Pages
1595-600
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC341785
Subset
IM
Grants
NIMH NIH HHS · P50 MH064045 · United States
NICHD NIH HHS · HD 37032 · United States
NIMH NIH HHS · P50 MH 64045 · United States
External Links
PubMed source DOI: 10.1073/pnas.0306846101 PMC Full Text View journal details
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