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PMID: 1494345 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

STE50, a novel gene required for activation of conjugation at an early step in mating in Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 236 ·No. 1 ·1992-12-00 ·Pages 145-54

Rad MR, Xu G, Hollenberg CP

Abstract

A new gene, STE50, which plays an essential role in cell differentiation in Saccharomyces cerevisiae was detected and analysed. STE50 expression is not cell type-specific and its expression in MATa and MAT alpha cells is unaffected by pheromones. When present on a high copy number plasmid, STE50 causes supersensitivity to alpha-pheromone, and increases the level of alpha-pheromone-induced transcription of FUS1 in haploid a cells. Mutants bearing either of the two gene disruptions, ste50-1 or ste50-2, are sterile and have a modulated sensitivity to alpha-pheromone. The overexpression of STE4 (G beta) in wild-type cells elicits a constitutive growth arrest signal, however this phenotype is suppressed by a C-terminal truncation mutation in STE50 (ste50-2). In contrast, the constitutive activation of the pheromone response pathway caused by disruption of GPA1 (G alpha) is not suppressed in ste50-2 mutants. The ste50-2 mutation partially suppresses the desensitisation defect of the sst2-1 mutation, and the resulting ste50-2 sst2-1 mutants restore fertility. Our results indicate that the ste50-2 mutant may have a defect in adaptation (hyperadaptation), and suggest a possible interaction of STE50-2 with the G alpha subunit of the G protein.

Related Genes
FUS1 GPA1 HIS3 SST2 STE50 URA3
MeSH Terms
Amino Acid Sequence Base Sequence Conjugation, Genetic DNA, Recombinant Fungal Proteins/genetics GTP-Binding Protein beta Subunits GTP-Binding Proteins/genetics,metabolism Gene Expression Genes, Fungal Heterotrimeric GTP-Binding Proteins Molecular Sequence Data Mutation Pheromones/metabolism Saccharomyces cerevisiae/cytology Saccharomyces cerevisiae Proteins
Chemicals
DNA, Recombinant Fungal Proteins GTP-Binding Protein beta Subunits Pheromones STE50 protein, S cerevisiae Saccharomyces cerevisiae Proteins Ste4 protein, S cerevisiae GTP-Binding Proteins Heterotrimeric GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rad M R
Institut für Mikrobiologie, Heinrich-Heine-Universität, Düsseldorf 1, FRG.
Xu G
Hollenberg C P
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00279653
Published
1992-12-00
Pages
145-54
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Databases
GENBANK
Z11116
External Links
PubMed source DOI: 10.1007/BF00279653
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