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PMID: 1495975 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tissue- and site-specific DNA recombination in transgenic mice.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 15 ·1992-08-01 ·Pages 6861-5

Orban PC, Chui D, Marth JD

Abstract

We have developed a method of specifically modifying the mammalian genome in vivo. This procedure comprises heritable tissue-specific and site-specific DNA recombination as a function of recombinase expression in transgenic mice. Transgenes encoding the bacteriophage P1 Cre recombinase and the loxP-flanked beta-galactosidase gene were used to generate transgenic mice. Genomic DNA from doubly transgenic mice exhibited tissue-specific DNA recombination as a result of Cre expression. Further characterization revealed that this process was highly efficient at distinct chromosomal integration sites. These studies also imply that Cre-mediated recombination provides a heritable marker for mitoses following the loss of Cre expression. This transgene-recombination system permits unique approaches to in vivo studies of gene function within experimentally defined spatial and temporal boundaries.

Related Genes
loxP
MeSH Terms
Animals Blotting, Southern CD4 Antigens/genetics CD8 Antigens/genetics Coliphages/enzymology,genetics DNA/genetics,isolation & purification DNA Nucleotidyltransferases/genetics,metabolism DNA Probes Growth Hormone/genetics Humans Integrases Mice Mice, Transgenic/genetics Organ Specificity Recombination, Genetic Restriction Mapping T-Lymphocytes/immunology,physiology Transcription, Genetic Viral Proteins beta-Galactosidase/genetics,metabolism
Chemicals
CD4 Antigens CD8 Antigens DNA Probes Viral Proteins Growth Hormone DNA Cre recombinase DNA Nucleotidyltransferases Integrases beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Orban P C
Biomedical Research Centre, University of British Columbia, Vancouver, Canada.
Chui D
Marth J D
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24 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.15.6861
Published
1992-08-01
Pages
6861-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49604
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.15.6861 PMC Full Text View journal details
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