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PMID: 1495994 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The budding yeast HRR25 gene product is a casein kinase I isoform.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 15 ·1992-08-01 ·Pages 7008-12

DeMaggio AJ, Lindberg RA, Hunter T, Hoekstra MF

Abstract

The Saccharomyces cerevisiae HRR25 gene was identified as a regulator of DNA strand-break repair. HRR25 encodes a protein kinase that is closely related to bovine casein kinase I (CKI). CKI is a ubiquitous multipotential protein kinase. Rabbit polyclonal antibodies that recognize and immunoprecipitate Hrr25p have been generated and an immune complex protein kinase assay has been developed. The reaction depends upon HRR25 and shows that Hrr25p uses casein as a substrate. The identity between Hrr25p and bovine CKI suggests that Hrr25p is a yeast isoform of the CKI family and that CKIs may play a role in regulating DNA metabolism.

Related Genes
HRR25
MeSH Terms
Amino Acid Sequence Animals Casein Kinases Caseins/isolation & purification,metabolism Cloning, Molecular Escherichia coli/genetics Genes, Fungal Isoenzymes/genetics,isolation & purification,metabolism Molecular Sequence Data Phosphorylation Phylogeny Protein Kinases/genetics,isolation & purification,metabolism Recombinant Proteins/isolation & purification,metabolism Saccharomyces cerevisiae/enzymology,genetics Sequence Homology, Nucleic Acid
Chemicals
Caseins Isoenzymes Recombinant Proteins Protein Kinases Casein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
DeMaggio A J
Molecular Biology and Virology Laboratory, Salk Institute, La Jolla, CA 92037.
Lindberg R A
Hunter T
Hoekstra M F
References (35)
35 references, click to expand
  1. Primary structure homology between the product of yeast cell division control gene CDC28 and vertebrate oncogenes.
    Nature. 1984 Jan 12-18;307(5947):183-5 PMID: 6361575
  2. Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2199-203 PMID: 6264467
  3. Pathways of ultraviolet mutability in Saccharomyces cerevisiae. III. Genetic analysis and properties of mutants resitant to ultraviolet-induced forward mutation.
    Mutat Res. 1977 May;43(2):179-204 PMID: 325400
  4. Two genes in Saccharomyces cerevisiae encode a membrane-bound form of casein kinase-1.
    Mol Biol Cell. 1992 Mar;3(3):275-86 PMID: 1627830
  5. Expression of the cDNA for the beta subunit of human casein kinase II confers partial UV resistance on xeroderma pigmentosum cells.
    Mutat Res. 1990 Jul;236(1):85-97 PMID: 1694965
  6. Novel yeast protein kinase (YPK1 gene product) is a 40-kilodalton phosphotyrosyl protein associated with protein-tyrosine kinase activity.
    Mol Cell Biol. 1990 Dec;10(12):6244-56 PMID: 1701015
  7. Yeast casein kinase I homologues: an essential gene pair.
    Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):28-32 PMID: 1729698
  8. Casein kinase II is a predominantly nuclear enzyme.
    J Cell Biol. 1992 Jan;116(1):43-55 PMID: 1730748
  9. Genetically identified protein kinases in yeast. I: Transcription, translation, transport and mating.
    Trends Genet. 1991 Aug;7(8):256-61 PMID: 1771673
  10. CTD kinase large subunit is encoded by CTK1, a gene required for normal growth of Saccharomyces cerevisiae.
    Gene Expr. 1991 May;1(2):149-67 PMID: 1820212
  11. HRR25, a putative protein kinase from budding yeast: association with repair of damaged DNA.
    Science. 1991 Aug 30;253(5023):1031-4 PMID: 1887218
  12. Spk1, a new kinase from Saccharomyces cerevisiae, phosphorylates proteins on serine, threonine, and tyrosine.
    Mol Cell Biol. 1991 Feb;11(2):987-1001 PMID: 1899289
  13. Purification of casein kinase I and isolation of cDNAs encoding multiple casein kinase I-like enzymes.
    Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9548-52 PMID: 1946367
  14. A novel protein kinase homolog essential for protein sorting to the yeast lysosome-like vacuole.
    Cell. 1991 Jan 25;64(2):425-37 PMID: 1988155
  15. Genetically identified protein kinases in yeast. II: DNA metabolism and meiosis.
    Trends Genet. 1991 Sep;7(9):293-7 PMID: 1763427
  16. Shuttle mutagenesis: bacterial transposons for genetic manipulations in yeast.
    Methods Enzymol. 1991;194:329-42 PMID: 1848645
  17. The cell-cycle-regulated budding yeast gene DBF2, encoding a putative protein kinase, has a homologue that is not under cell-cycle control.
    Gene. 1991 Jul 31;104(1):63-70 PMID: 1916278
  18. Protein kinase catalytic domain sequence database: identification of conserved features of primary structure and classification of family members.
    Methods Enzymol. 1991;200:38-62 PMID: 1956325
  19. High-expression vectors with multiple cloning sites for construction of trpE fusion genes: pATH vectors.
    Methods Enzymol. 1991;194:477-90 PMID: 2005804
  20. cDNA cloning and characterization of eck, an epithelial cell receptor protein-tyrosine kinase in the eph/elk family of protein kinases.
    Mol Cell Biol. 1990 Dec;10(12):6316-24 PMID: 2174105
  21. The product of the Saccharomyces cerevisiae cell cycle gene DBF2 has homology with protein kinases and is periodically expressed in the cell cycle.
    Mol Cell Biol. 1990 Apr;10(4):1358-66 PMID: 2181271
  22. Platelet-derived growth factor induces multisite phosphorylation of pp60c-src and increases its protein-tyrosine kinase activity.
    Mol Cell Biol. 1988 Aug;8(8):3345-56 PMID: 2463476
  23. A newly synthesized selective casein kinase I inhibitor, N-(2-aminoethyl)-5-chloroisoquinoline-8-sulfonamide, and affinity purification of casein kinase I from bovine testis.
    J Biol Chem. 1989 Mar 25;264(9):4924-7 PMID: 2925675
  24. CDC7-dependent protein kinase activity in yeast replicative-complex preparations.
    Proc Natl Acad Sci U S A. 1988 Apr;85(7):2101-5 PMID: 3281161
  25. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 PMID: 6246487
  26. Casein kinase I and II--multipotential serine protein kinases: structure, function, and regulation.
    Adv Second Messenger Phosphoprotein Res. 1991;23:123-64 PMID: 1997039
  27. Multiple Ca2+/calmodulin-dependent protein kinase genes in a unicellular eukaryote.
    EMBO J. 1991 Jun;10(6):1511-22 PMID: 2026147
  28. Preparation of extracts from yeast.
    Methods Enzymol. 1990;182:154-74 PMID: 2179664
  29. Isolation, sequencing, and disruption of the yeast CKA2 gene: casein kinase II is essential for viability in Saccharomyces cerevisiae.
    Mol Cell Biol. 1990 Aug;10(8):4089-99 PMID: 2196445
  30. Protein blotting and immunodetection.
    Methods Enzymol. 1990;182:679-88 PMID: 2314263
  31. Regulation of the RAD2 gene of Saccharomyces cerevisiae.
    Mol Microbiol. 1989 Dec;3(12):1697-707 PMID: 2695743
  32. Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase.
    Cell. 1987 Jul 17;50(2):277-87 PMID: 3036373
  33. Isolation, sequencing, and disruption of the CKA1 gene encoding the alpha subunit of yeast casein kinase II.
    Mol Cell Biol. 1988 Nov;8(11):4981-90 PMID: 3062376
  34. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Methods Enzymol. 1987;154:367-82 PMID: 3323813
  35. Purification and properties of a yeast protein kinase.
    Biochemistry. 1975 May 6;14(9):2015-23 PMID: 235975
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.15.7008
Published
1992-08-01
Pages
7008-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49634
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.15.7008 PMC Full Text View journal details
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