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PMID: 1495999 Published · ppublish English Journal Article

Molecular basis of the membrane-anchored and two soluble isoforms of the human interleukin 5 receptor alpha subunit.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 15 ·1992-08-01 ·Pages 7041-5

Tavernier J, Tuypens T, Plaetinck G, Verhee A, Fiers W, Devos R

Abstract

By use of a 3' extension PCR strategy, cDNA clones were isolated spanning the transmembrane region and a complete cytoplasmic domain of the human interleukin 5 receptor alpha subunit (hIL5R alpha). These cDNAs differ from previously isolated clones encoding a soluble hIL5R alpha form by a sequence switch at position 1243. When expressed in COS-1 cells, only low-affinity binding of 125I-labeled human interleukin 5 was observed. Coexpression of the hIL5R beta chain led to a 2-fold increase in binding affinity. In addition, this same cloning strategy allowed us to identify a putative second soluble isoform of hIL5R alpha. Genomic data revealed that the two soluble variants arise from either a "normal" splicing event or from the absence of splicing, whereas synthesis of the membrane-anchored form requires alternative splicing.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Cell Membrane/immunology DNA/genetics Female Genomic Library Humans Interleukin-5/metabolism Kinetics Macromolecular Substances Molecular Sequence Data Placenta/physiology Polymerase Chain Reaction/methods Pregnancy RNA Splicing RNA, Messenger/genetics Receptors, Immunologic/genetics,metabolism Receptors, Interleukin Receptors, Interleukin-5 Transfection
Chemicals
Interleukin-5 Macromolecular Substances RNA, Messenger Receptors, Immunologic Receptors, Interleukin Receptors, Interleukin-5 DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tavernier J
Roche Research Gent, Belgium.
Tuypens T
Plaetinck G
Verhee A
Fiers W
Devos R
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.15.7041
Published
1992-08-01
Pages
7041-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC49641
Subset
IM
Databases
GENBANK
M96651, M96652
External Links
PubMed source DOI: 10.1073/pnas.89.15.7041 PMC Full Text View journal details
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