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PMID: 14973166 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Type-A Arabidopsis response regulators are partially redundant negative regulators of cytokinin signaling.

The Plant cell ·Vol. 16 ·No. 3 ·2004-03-00 ·Pages 658-71

To JP, Haberer G, Ferreira FJ, Deruère J, Mason MG, Schaller GE, Alonso JM, Ecker JR, Kieber JJ

Abstract

Type-A Arabidopsis (Arabidopsis thaliana) response regulators (ARRs) are a family of 10 genes that are rapidly induced by cytokinin and are highly similar to bacterial two-component response regulators. We have isolated T-DNA insertions in six of the type-A ARRs and constructed multiple insertional mutants, including the arr3,4,5,6,8,9 hextuple mutant. Single arr mutants were indistinguishable from the wild type in various cytokinin assays; double and higher order arr mutants showed progressively increasing sensitivity to cytokinin, indicating functional overlap among type-A ARRs and that these genes act as negative regulators of cytokinin responses. The induction of cytokinin primary response genes was amplified in arr mutants, indicating that the primary response to cytokinin is affected. Spatial patterns of ARR gene expression were consistent with partially redundant function of these genes in cytokinin signaling. The arr mutants show altered red light sensitivity, suggesting a general involvement of type-A ARRs in light signal transduction. Further, morphological phenotypes of some arr mutants suggest complex regulatory interactions and gene-specific functions among family members.

MeSH Terms
Arabidopsis/drug effects,genetics,growth & development,metabolism Arabidopsis Proteins/genetics,metabolism Cytokinins/metabolism,pharmacology DNA Transposable Elements DNA, Bacterial/genetics DNA-Binding Proteins/genetics,metabolism Genes, Plant/drug effects Light Mutation Phenotype Phylogeny Plant Roots/growth & development Seedlings/growth & development Signal Transduction Transcription Factors/genetics,metabolism
Chemicals
Arabidopsis Proteins Cytokinins DNA Transposable Elements DNA, Bacterial DNA-Binding Proteins T-DNA Transcription Factors
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
To Jennifer P C
Department of Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA.
Haberer Georg
Ferreira Fernando J
Deruère Jean
Mason Michael G
Schaller G Eric
Alonso Jose M
Ecker Joseph R
Kieber Joseph J
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.018978
Published
2004-03-00
Epub
2004-00-18
Pages
658-71
Language
English
Region
England
NLM ID
9208688
PMCID
PMC385279
Subset
IM
Grants
NIGMS NIH HHS · R01 GM064425-04 · United States
External Links
PubMed source DOI: 10.1105/tpc.018978 PMC Full Text View journal details
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