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PMID: 1497607 Published · ppublish English Comparative Study Journal Article

Anti-(14-3-3 protein) antibody inhibits stimulation of noradrenaline (norepinephrine) secretion by chromaffin-cell cytosolic proteins.

The Biochemical journal ·Vol. 285 ( Pt 3) ·1992-08-01 ·Pages 697-700

Wu YN, Vu ND, Wagner PD

Abstract

Incubation of digitonin-permeabilized bovine chromaffin cells in the absence of Ca2+ results in a loss of both cytosolic proteins and Ca(2+)-dependent secretion. Addition of these leaked proteins prevents this loss of secretory activity. We have purified a protein from an extract of bovine adrenal medulla which can partially prevent this loss of Ca(2+)-dependent secretion. Antibody against this protein inhibited the ability of leaked chromaffin-cell proteins to prevent the loss of Ca(2+)-dependent secretion. Sequence analysis showed it to have sequence identity with bovine brain 14-3-3 protein. These results demonstrate that 14-3-3 protein makes a significant contribution to the ability of leaked chromaffin-cell proteins to maintain secretory activity.

MeSH Terms
14-3-3 Proteins Adrenal Medulla/chemistry,metabolism,ultrastructure Amino Acid Sequence Animals Antibodies Brain Chemistry Calcium/pharmacology Cattle Cells, Cultured Chromaffin System/chemistry,metabolism,ultrastructure Cytosol/chemistry Molecular Sequence Data Nerve Tissue Proteins/chemistry,immunology,physiology Norepinephrine/metabolism Tyrosine 3-Monooxygenase
Chemicals
14-3-3 Proteins Antibodies Nerve Tissue Proteins Tyrosine 3-Monooxygenase Calcium Norepinephrine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wu Y N
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892.
Vu N D
Wagner P D
References (26)
26 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2850697
Published
1992-08-01
Pages
697-700
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1132849
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2850697 PMC Full Text View journal details
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