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PMID: 14981095 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Epiblast cells that express MyoD recruit pluripotent cells to the skeletal muscle lineage.

The Journal of cell biology ·Vol. 164 ·No. 5 ·2004-03-01 ·Pages 739-46

Gerhart J, Neely C, Stewart B, Perlman J, Beckmann D, Wallon M, Knudsen K, George-Weinstein M

Abstract

Embryonic stem cells are derived from the epiblast. A subpopulation of epiblast cells expresses MyoD mRNA and the G8 antigen in vivo. G8 positive (G8pos) and G8 negative (G8neg) populations were isolated by magnetic cell sorting. Nearly all G8pos cells switched from E- to N-cadherin and differentiated into skeletal muscle in culture. G8neg cells were impaired in their ability to switch cadherins and few formed skeletal muscle. Medium conditioned by G8pos cells stimulated skeletal myogenesis and N-cadherin synthesis in G8neg cultures. The effect of conditioned medium from G8pos cultures was inhibited by bone morphogenetic protein (BMP) 4. Treatment of G8neg cells with a soluble form of the BMP receptor-IA or Noggin promoted N-cadherin synthesis and skeletal myogenesis. These results demonstrate that MyoD-positive epiblast cells recruit pluripotent cells to the skeletal muscle lineage. The mechanism of recruitment involves blocking the BMP signaling pathway.

MeSH Terms
Animals Biomarkers Bone Morphogenetic Protein 4 Bone Morphogenetic Protein Receptors, Type I Bone Morphogenetic Proteins/metabolism Cadherins/metabolism Carrier Proteins Cell Differentiation/physiology Cell Lineage Cells, Cultured Chick Embryo/anatomy & histology,physiology Culture Media, Conditioned Cytoskeletal Proteins/metabolism Epithelium/anatomy & histology,physiology Immunomagnetic Separation Muscle, Skeletal/cytology,embryology MyoD Protein/genetics,metabolism Pluripotent Stem Cells/cytology,physiology Protein Serine-Threonine Kinases/metabolism Proteins/metabolism Receptors, Growth Factor/metabolism Trans-Activators/metabolism beta Catenin
Chemicals
Biomarkers Bone Morphogenetic Protein 4 Bone Morphogenetic Proteins Cadherins Carrier Proteins Culture Media, Conditioned Cytoskeletal Proteins MyoD Protein Proteins Receptors, Growth Factor Trans-Activators beta Catenin noggin protein Protein Serine-Threonine Kinases Bone Morphogenetic Protein Receptors, Type I
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Gerhart Jacquelyn
Department of Anatomy, Philadelphia College of Osteopathic Medicine, 4170 City Ave., Philadelphia, PA 19131, USA.
Neely Christine
Stewart Benjamin
Perlman Jordanna
Beckmann David
Wallon Margaretha
Knudsen Karen
George-Weinstein Mindy
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200309152
Published
2004-03-01
Epub
2004-00-23
Pages
739-46
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC1615912
Subset
IM
Grants
NIGMS NIH HHS · R01 GM061702 · United States
NICHD NIH HHS · R01 HD043157 · United States
NIGMS NIH HHS · GM61702 · United States
NICHD NIH HHS · HD043157 · United States
External Links
PubMed source DOI: 10.1083/jcb.200309152 PMC Full Text View journal details
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