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PMID: 1498363 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Actin- and tubulin-dependent functions during Saccharomyces cerevisiae mating projection formation.

Molecular biology of the cell ·Vol. 3 ·No. 4 ·1992-04-00 ·Pages 429-44

Read EB, Okamura HH, Drubin DG

Abstract

Several conditional-lethal mutant alleles of the single-copy Saccharomyces cerevisiae beta-tubulin and actin genes were used to evaluate the roles of microtubules and actin filaments in the pheromone-induced extension of mating projections. Mutants defective in tubulin assembly form projections indistinguishable in appearance from those formed by wild-type cells. However, the tubulin mutants are unable to move their nuclei into the projections and to orient the spindle pole body associated with each nucleus toward the projection tip. Actin mutants are defective in spatial orientation of cell-surface growth required for formation of normal mating projections. Migration of nuclei into mating projections and Spa2p segregation to projection tips are also defective in actin mutants. Studies with abp1 null mutants showed that the function of the Abp1p actin-binding protein is either not required for projection formation or there are other proteins in yeast with similar functions. Our findings demonstrate that actin is required to restrict cell-surface growth to a defined region for pheromone-induced morphogenesis and suggest that nuclear position and orientation in mating projections depend on direct or indirect interaction of microtubules with actin filaments.

MeSH Terms
Actins/genetics,metabolism Cell Nucleus/ultrastructure Cell Polarity/physiology Conjugation, Genetic/drug effects,physiology Cytoskeleton/metabolism Fluorescent Antibody Technique Microtubules/metabolism Morphogenesis Mutation Saccharomyces cerevisiae/cytology,genetics,physiology Sex Attractants/pharmacology Tubulin/genetics,metabolism
Chemicals
Actins Sex Attractants Tubulin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Read E B
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Okamura H H
Drubin D G
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.3.4.429
Published
1992-04-00
Pages
429-44
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC275593
Subset
IM
Grants
NIGMS NIH HHS · GM-42759 · United States
External Links
PubMed source DOI: 10.1091/mbc.3.4.429 PMC Full Text View journal details
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